Moazzem Hossen S M, Akramul Hoque Tanim Mohammad, Shahadat Hossain Mohammad, Ahmed Sami Saad, Uddin Emon Nazim
Department of Pharmacy, Faculty of Biological Science, University of Chittagong, Chittagong 4331, Bangladesh.
Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong 4318, Bangladesh.
Saudi J Biol Sci. 2021 Nov;28(11):6375-6383. doi: 10.1016/j.sjbs.2021.07.016. Epub 2021 Jul 10.
The present study sought to evaluate the central nervous system (CNS) depressant, antioxidant, and cytotoxicity activity of methanol and aqueous extract of Trametes versicolor (METV and AETV). The CNS activity was assessed by the open field, hole-cross, forced swimming, thiopental sodium-induced sleeping time, hole-board, and rotarod tests in Swiss albino mice. For both extracts, a substantial decrease in locomotion was observed in open field and hole-cross tests. In addition, the molecular docking study has been implemented through Maestro V11.1. The higher dose of METV (400 mg/kg) and the lower dose of AETV (200 mg/kg) exhibited a significant decrease in immobility time in forced swimming test and increased prolongation of sleep in thiopental sodium-induced sleeping time test, respectively. In contrast, a moderate finding was observed for the hole-board and rotarod tests. Additionally, a significant DPPH scavenging assay and a high toxicity effect in brine shrimp lethality assay were observed. Besides, five phenolic compounds, namely baicalin, quercetin, catechin, p-hydroxybenzoic acid, and quinic acid, were used for the molecular docking study, whereas catechin demonstrated the highest binding affinity towards the targets. The findings conclude that the could be an alternative source for CNS anti-depressant and antioxidant activity.
本研究旨在评估云芝甲醇提取物和水提取物（METV和AETV）的中枢神经系统（CNS）抑制、抗氧化及细胞毒性活性。通过旷场试验、穿洞试验、强迫游泳试验、硫喷妥钠诱导睡眠时间试验、跳台试验和转棒试验对瑞士白化小鼠的中枢神经系统活性进行评估。对于两种提取物，在旷场试验和穿洞试验中均观察到运动能力显著下降。此外，通过Maestro V11.1进行了分子对接研究。较高剂量的METV（400 mg/kg）和较低剂量的AETV（200 mg/kg）分别在强迫游泳试验中使不动时间显著减少，在硫喷妥钠诱导睡眠时间试验中使睡眠时间延长。相比之下，跳台试验和转棒试验的结果中等。此外，在DPPH清除试验中观察到显著效果，在卤虫致死试验中观察到高毒性作用。此外，使用了五种酚类化合物，即黄芩苷、槲皮素、儿茶素、对羟基苯甲酸和奎尼酸进行分子对接研究，其中儿茶素对靶点表现出最高的结合亲和力。研究结果表明，[提取物名称未给出]可能是中枢神经系统抗抑郁和抗氧化活性的替代来源。
