可见光或砷化镓激光与不同光敏剂联合对牙龈卟啉单胞菌和具核梭杆菌的光毒性作用

Synergic phototoxic effect of visible light or Gallium-Arsenide laser in the presence of different photo-sensitizers on Porphyromonas gingivalis and Fusobacterium nucleatum.

作者信息

Ghanbari Habibollah, Mousavi Seyed Amir, Forouzanfar Ali, Zakeri Mahdi, Shafaee Hooman, Shahnaseri Shirin

机构信息

Oral and Maxillofacial Diseases Research Center and Department of Periodontics, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.

Torabinejad Dental Research Center and Departments of Endodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Dent Res J (Isfahan). 2015 Jul-Aug;12(4):323-30. doi: 10.4103/1735-3327.161432.

DOI:10.4103/1735-3327.161432

PMID:26288621

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4533189/

Abstract

BACKGROUND

According to the development of resistant strains of pathogenic bacteria following treatment with antimicrobial chemotherapeutic agents, alternative approaches such as lethal photosensitization are being used. The aim of this study was to evaluate the effect of visible light and laser beam radiation in conjugation with three different photosensitizers on the survival of two main periodontopathogenic bacteria including Porphyromonas gingivalis and Fusobacterium nucleatum in different exposure periods.

MATERIALS AND METHODS

In this in vitro prospective study, strains of P. gingivalis and F. nucleatum. were exposed to visible light at wavelengths of 440 nm and diode laser light, Gallium-Arsenide, at wavelength of 830 nm in the presence of a photosensitizer (erythrosine, curcuma, or hydrogen peroxide). They were exposed 1-5 min to each light. Each experiment was repeated 3 times for each strain of bacteria. Data were analyzed by two-ways ANOVA and least significant difference post-hoc tests. P < 0.05 was considered as significant. After 4 days the colonies were counted.

RESULTS

Viability of P. gingivalis was reduced 10% and 20% subsequent to exposure to visible light and diode laser, respectively. The values were 65% and 75% for F. nucleatum in a period of 5-min, respectively. Exposure to visible light or laser beam in conjugation with the photosensitizers suspension caused significant reduction in the number of P. gingivalis in duration of 5-min, suggesting a synergic phototoxic effect. However, the survival rate of F. nucleatum following the exposure to laser with hydrogen peroxide, erythrosine and rhizome of Curcuma longa (curcumin) after 5-min was 10%, 20% and 90% respectively.

CONCLUSION

Within the limitations of this study, the synergic phototoxic effect of visible light in combination with each of the photosensitizers on P. gingivalis and F. nucleatum. However, the synergic phototoxic effect of laser exposure and hydrogen peroxide and curcumin as photosensitizers on F. nucleatum was not shown.

摘要

背景

随着抗菌化疗药物治疗后病原菌耐药菌株的出现，诸如致死性光致敏等替代方法正在被采用。本研究的目的是评估可见光和激光束辐射与三种不同光敏剂结合，在不同暴露时间段对两种主要牙周病原菌（牙龈卟啉单胞菌和具核梭杆菌）存活的影响。

材料与方法

在这项体外前瞻性研究中，牙龈卟啉单胞菌和具核梭杆菌菌株在光敏剂（赤藓红、姜黄或过氧化氢）存在的情况下，分别暴露于波长为440nm的可见光和波长为830nm的二极管激光（砷化镓）下。它们分别暴露于每种光下1 - 5分钟。每种细菌菌株的每个实验重复3次。数据通过双向方差分析和最小显著差异事后检验进行分析。P < 0.05被认为具有显著性。4天后对菌落进行计数。

结果

牙龈卟啉单胞菌在暴露于可见光和二极管激光后，存活率分别降低了10%和20%。具核梭杆菌在5分钟时间段内的值分别为65%和75%。与光敏剂悬浮液结合暴露于可见光或激光束会导致牙龈卟啉单胞菌数量在5分钟持续时间内显著减少，表明存在协同光毒性作用。然而，具核梭杆菌在暴露于激光与过氧化氢、赤藓红和姜黄（姜黄素）5分钟后的存活率分别为10%、20%和90%。