Sigusch Bernd W, Pfitzner Anne, Albrecht Volker, Glockmann Eike
Friedrich-Schiller-University of Jena, Clinic for Conservative Dentistry, An der alten Post 4, 07740 Jena, Germany.
J Periodontol. 2005 Jul;76(7):1100-5. doi: 10.1902/jop.2005.76.7.1100.
Current research aims to find alternatives to conventional methods for suppressing periodontopathogenic bacteria. Photodynamic therapy (PDT) could be a suitable treatment procedure of periodontal infections.
In the present study, the PDT method was tested with two photosensitizers, chlorine e6 and BLC1010, in an experiment on beagle dogs. The animals were infected with Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) in all subgingival areas. After infection, we observed clinical signs of gingival inflammation, including an increase of redness and bleeding on probing. Microbiological monitoring before and after treatment was performed using polymerase chain reaction (PCR). PDT was conducted with a diode laser with a wavelength of 662 nm using a power of 0.5 W and the photosensitizers.
The PDT procedure carried out with either of the photosensitizers caused a significant reduction in the clinical inflammation signs of redness and BOP, compared to the controls (laser only and no treatment). Furthermore, PDT with chlorine e6 caused a significant reduction in P. gingivalis-infected sites, whereas there was a lack in suppression after PDT with BLC1010. F. nucleatum could hardly be reduced with chlorine e6, and only to a certain extent with BLC 1010 and laser only. In the control groups, the Pg-infected test sites did not change.
This study demonstrated that the photodynamic therapy using photosensitizer and a 662 nm laser light source is distinctly advantageous in reducing the periodontal signs of redness and bleeding on probing. The procedure also appears to significantly suppress P. gingivalis.
当前研究旨在寻找传统方法的替代方案以抑制牙周病原菌。光动力疗法（PDT）可能是一种合适的牙周感染治疗方法。
在本研究中，在比格犬实验中使用两种光敏剂（氯e6和BLC1010）测试PDT方法。所有龈下区域均感染牙龈卟啉单胞菌（Pg）和具核梭杆菌（Fn）。感染后，观察牙龈炎症的临床体征，包括探诊时发红和出血增加。使用聚合酶链反应（PCR）进行治疗前后的微生物监测。使用波长为662nm、功率为0.5W的二极管激光和光敏剂进行PDT。
与对照组（仅激光照射和不治疗）相比，使用任一种光敏剂进行的PDT程序均导致发红和探诊出血的临床炎症体征显著减轻。此外，使用氯e6进行的PDT导致牙龈卟啉单胞菌感染部位显著减少，而使用BLC1010进行PDT后缺乏抑制作用。使用氯e6几乎无法减少具核梭杆菌，使用BLC1010和仅激光照射只能在一定程度上减少。在对照组中，Pg感染的测试部位没有变化。
本研究表明，使用光敏剂和662nm激光光源的光动力疗法在减少探诊时的牙周发红和出血体征方面具有明显优势。该程序似乎还能显著抑制牙龈卟啉单胞菌。
