Feuerstein Osnat, Persman Nir, Weiss Ervin I
Department of Prosthodontics, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
Photochem Photobiol. 2004 Nov-Dec;80(3):412-5. doi: 10.1562/0031-8655(2004)080<0412:PEOVLO>2.0.CO;2.
The antibacterial effect of visible light irradiation combined with photosensitizers has been reported. The objective of this was to test the effect of visible light irradiation without photosensitizers on the viability of oral microorganisms. Strains of Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus mutans and Streptococcus faecalis in suspension or grown on agar were exposed to visible light at wavelengths of 400-500 nm. These wavelengths are used to photopolymerize composite resins widely used for dental restoration. Three photocuring light sources, quartz-tungsten-halogen lamp, light-emitting diode and plasma-arc, at power densities between 260 and 1300 mW/cm2 were used for up to 3 min. Bacterial samples were also exposed to a near-infrared diode laser (wavelength, 830 nm), using identical irradiation parameters for comparison. The results show that blue light sources exert a phototoxic effect on P. gingivalis and F. nucleatum. The minimal inhibitory dose for P. gingivalis and F. nucleatum was 16-62 J/cm2, a value significantly lower than that for S. mutans and S. faecalis (159-212 J/cm2). Near-infrared diode laser irradiation did not affect any of the bacteria tested. Our results suggest that visible light sources without exogenous photosensitizers have a phototoxic effect mainly on Gram-negative periodontal pathogens.
可见光照射联合光敏剂的抗菌作用已有报道。本研究的目的是测试无光敏剂的可见光照射对口腔微生物活力的影响。将悬浮状态或在琼脂上生长的牙龈卟啉单胞菌、具核梭杆菌、变形链球菌和粪肠球菌菌株暴露于波长为400 - 500 nm的可见光下。这些波长被广泛用于牙科修复用复合树脂的光聚合反应。使用三种光固化光源，即石英 - 钨 - 卤素灯、发光二极管和等离子弧，功率密度在260至1300 mW/cm²之间，照射时间长达3分钟。细菌样本还暴露于近红外二极管激光（波长830 nm）下，使用相同的照射参数进行比较。结果表明，蓝光光源对牙龈卟啉单胞菌和具核梭杆菌具有光毒性作用。牙龈卟啉单胞菌和具核梭杆菌的最小抑菌剂量为16 - 62 J/cm²，该值显著低于变形链球菌和粪肠球菌（159 - 212 J/cm²）。近红外二极管激光照射对所测试的任何细菌均无影响。我们的结果表明，无外源性光敏剂的可见光光源主要对革兰氏阴性牙周病原体具有光毒性作用。
