Avci Oguzhan, Bulut Oya, Dik Irmak
Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42003 Konya, Turkey.
J Vet Med Sci. 2016 Jan;78(1):129-31. doi: 10.1292/jvms.15-0231. Epub 2015 Aug 20.
The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intraperitoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.
本研究的目的是确定腹腔注射异丙酚对大鼠促炎和抗炎细胞因子水平的影响。除7只大鼠（对照组，0组）外，对49只大鼠经腹腔途径给予腹腔注射异丙酚（1 ml/大鼠）。在治疗后的第1、2、4、8、12、16和24小时从7只大鼠采集血清样本。使用酶联免疫吸附测定法测定肿瘤坏死因子-α、白细胞介素-6、白细胞介素-12和白细胞介素-10的水平。腹腔注射异丙酚刺激肿瘤坏死因子-α（第16和24小时）和白细胞介素-6（第12、16和24小时）的合成，并导致白细胞介素-10和白细胞介素-12浓度波动。总之，细胞因子水平升高可能归因于腹腔注射异丙酚的免疫调节活性，然而，需要进行详细研究以充分了解腹腔注射异丙酚对免疫系统的影响。
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