感染猴免疫缺陷病毒前后雌性恒河猴生殖微环境的特征分析
Characterization of the Genital Microenvironment of Female Rhesus Macaques Prior to and After SIV Infection.
作者信息
Nichols Whitney A, Birke Leslie, Dufour Jason, Loganantharaj Nisha, Bagby Gregory J, Nelson Steve, Molina Patricia E, Amedee Angela M
机构信息
Department of Microbiology, Immunology, & Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
Division of Animal Care, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
出版信息
Am J Reprod Immunol. 2015 Dec;74(6):508-22. doi: 10.1111/aji.12422. Epub 2015 Aug 20.
PROBLEM
HIV infection among women is frequently modeled in female rhesus macaques. Longitudinal studies on genital compartment and hormonal factors that can influence susceptibility to SIV infection are lacking in this animal model.
METHOD OF STUDY
Genital specimens and menstruation of indoor-housed female rhesus macaques were analyzed prior to and after SIV infection.
RESULTS
Median menstrual cycle length averaged 27 days, although highly variable cycle lengths and frequent periods of amenorrhea were observed during summer months. The vaginal microbiota, characterized by adapted Nugent scoring, showed predominance of small Gram-variable rods and Gram-positive cocci. Highly variable vaginal cytokine levels were observed pre- and post-SIV infection. Vaginal viral loads correlated with plasma viral loads, but were not associated with progesterone levels.
CONCLUSION
These results provide an integrated characterization of important factors in the vaginal microenvironment that are relevant to the experimental design of HIV prevention and transmission studies in female rhesus macaques.
问题
女性中的HIV感染常以雌性恒河猴作为模型进行研究。在此动物模型中，缺乏关于可影响SIV感染易感性的生殖系统因素和激素因素的纵向研究。
研究方法
对圈养雌性恒河猴在感染SIV之前和之后的生殖系统标本及月经情况进行分析。
结果
月经周期中位数平均为27天，不过在夏季观察到周期长度高度可变且经常出现闭经。通过改良的纽金特评分法表征的阴道微生物群显示，小型革兰氏可变杆菌和革兰氏阳性球菌占优势。在感染SIV前后观察到阴道细胞因子水平高度可变。阴道病毒载量与血浆病毒载量相关，但与孕酮水平无关。
结论
这些结果提供了与雌性恒河猴HIV预防和传播研究的实验设计相关的阴道微环境中重要因素的综合特征。
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