二氧化硅在体外促进大鼠循环纤维细胞的转分化。
Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro.
作者信息
Yao Wu, Liu Suna, Li Ju, Hao Changfu
机构信息
Department of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
出版信息
Mol Med Rep. 2015 Oct;12(4):5828-36. doi: 10.3892/mmr.2015.4212. Epub 2015 Aug 11.
To investigate the effects of silica on circulating fibrocytes (cFbs), the present study established a primary culture model of rat alveolar macrophages and cFbs in vitro. Macrophages were treated with free silica, and their supernatant was used to stimulate cFbs. The mRNA expression levels of collagen I, collagen III and α‑smooth muscle actin (SMA) in cFbs were analyzed by reverse transcription‑quantitative polymerase chain reaction. The intracellular and extracellular protein expression levels of collagen I, collagen III and α‑SMA were detected by ELISA and immunofluorescence staining. The results indicated that in the cell model, the free silica effectively increased the protein and mRNA expression levels of collagen‑I, collagen‑III and α‑SMA. The free silica significantly promoted the transdifferentiation of cFbs into myofibroblasts in a dose‑and time-dependent manner.
为研究二氧化硅对循环纤维细胞（cFbs）的影响，本研究建立了大鼠肺泡巨噬细胞和cFbs的体外原代培养模型。用游离二氧化硅处理巨噬细胞，并用其培养上清液刺激cFbs。通过逆转录-定量聚合酶链反应分析cFbs中I型胶原、III型胶原和α-平滑肌肌动蛋白（SMA）的mRNA表达水平。通过酶联免疫吸附测定法和免疫荧光染色检测I型胶原、III型胶原和α-SMA的细胞内和细胞外蛋白表达水平。结果表明，在该细胞模型中，游离二氧化硅有效提高了I型胶原、III型胶原和α-SMA的蛋白和mRNA表达水平。游离二氧化硅以剂量和时间依赖性方式显著促进cFbs向肌成纤维细胞的转分化。
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