Bhat Sushant, Bhatia Sandeep, Pillai Aravind S, Sood Richa, Singh Vikas Kumar, Shrivas Om Prakash, Mishra Suchitra K, Mawale Namrata
ICAR-National Institute of High Security Animal Diseases (NIHSAD), Anand Nagar, Bhopal, MP 462021, India.
ICAR-National Institute of High Security Animal Diseases (NIHSAD), Anand Nagar, Bhopal, MP 462021, India.
Microb Pathog. 2015 Nov;88:87-93. doi: 10.1016/j.micpath.2015.08.010. Epub 2015 Aug 20.
The recurrent circulation of highly pathogenic avian influenza (HPAI) H5N1 in Indian poultry since 2006 resulted in emergence of the viruses of distinct antigenic clades of haemagglutinin (HA) with the majority of the H5N1 outbreaks since 2011 belonging to clade 2.3.2.1. The present study was aimed to characterize the antigenic profile of a collection of H5N1 HPAI viruses of clade 2.3.2.1 isolated in India by applying antigenic cartography, serological data and phylogenetic analysis. Eleven H5N1 viruses (2 of clade 2.2 and 9 of clade 2.3.2.1) were selected based on genetic analysis and were further characterized by antigenic cartography analysis based on cross HI (hemagglutination inhibition) data. This study highlights the intercladal antigenic differences between clades 2.3.2.1 and 2.2 and the intracladal antigenic divergence among the clade 2.3.2.1 viruses. Five viruses of clade 2.3.2.1 were also studied for analysis of glycosylation pattern of Hemagglutinin (HA) gene and the growth kinetics analysis in MDCK cells in which the viruses CL03485/H5N1 and 03CL488/H5N1 showed better replication kinetics than other viruses. The study presents a baseline data of antigenicity and other factors that can be used in the selection of suitable H5 vaccine strains or HA donor viruses to develop H5 vaccine strains by reverse genetics or other methods for control of currently circulating H5N1 viruses in Indian region.
自2006年以来，高致病性禽流感（HPAI）H5N1在印度家禽中反复循环传播，导致出现了血凝素（HA）不同抗原分支的病毒，2011年以来的大多数H5N1疫情都属于2.3.2.1分支。本研究旨在通过应用抗原图谱、血清学数据和系统发育分析，对在印度分离的2.3.2.1分支H5N1 HPAI病毒的抗原特性进行表征。基于遗传分析选择了11株H5N1病毒（2株属于2.2分支，9株属于2.3.2.1分支），并根据交叉血凝抑制（HI）数据，通过抗原图谱分析对其进行进一步表征。本研究突出了2.3.2.1分支和2.2分支之间的分支间抗原差异以及2.3.2.1分支病毒之间的分支内抗原差异。还对5株2.3.2.1分支病毒进行了血凝素（HA）基因糖基化模式分析以及在MDCK细胞中的生长动力学分析，其中CL03485/H5N1和03CL488/H5N1病毒比其他病毒表现出更好的复制动力学。该研究提供了抗原性及其他因素的基线数据，可用于选择合适的H5疫苗株或HA供体病毒，通过反向遗传学或其他方法来开发H5疫苗株，以控制印度地区当前流行的H5N1病毒。
