Huang Yinxi, Yang Hui Ying, Ai Ye
Pillar of Engineering Product Development, Singapore University of Technology and Design , Singapore 487372.
Anal Chem. 2015 Sep 15;87(18):9132-6. doi: 10.1021/acs.analchem.5b03037. Epub 2015 Sep 3.
Single nucleotide polymorphisms (SNPs) are frequently associated with various gene-related human diseases, whose determination has attracted great interest. Herein, we report a graphene oxide (GO) nanosheets-based fluorometric DNA biosensor to study the type and location of the single-base mismatch, as well as the influence of the length of the strands. The results indicated that both short and long targets led to much lower fluorescence signals than the perfectly complementary target, while the type of mismatched base had negligible influence on the results. Furthermore, targets with mismatch location near the 5' end led to higher fluorescence intensity than those near the 3' end when the dye was tagged at the 5' end of the probe.
单核苷酸多态性（SNPs）常与各种基因相关的人类疾病有关，其测定引起了人们极大的兴趣。在此，我们报道了一种基于氧化石墨烯（GO）纳米片的荧光DNA生物传感器，用于研究单碱基错配的类型和位置，以及链长度的影响。结果表明，与完全互补的靶标相比，短靶标和长靶标均导致荧光信号低得多，而错配碱基的类型对结果的影响可忽略不计。此外，当染料标记在探针的5'端时，错配位置靠近5'端的靶标比靠近3'端的靶标导致更高的荧光强度。
