鉴定和定量分析澳大利亚土薄荷（Mentha australis R. Br.）中的酚类物质。

Identification and quantification of phenolics in Australian native mint (Mentha australis R. Br.).

机构信息

Food Science and Technology, School of Chemical Engineering, UNSW Australia, Sydney 2052, Australia.

出版信息

Food Chem. 2016 Feb 1;192:698-705. doi: 10.1016/j.foodchem.2015.07.032. Epub 2015 Jul 17.

DOI:10.1016/j.foodchem.2015.07.032

Abstract

Australian native mints have traditionally been used by the aboriginal people for natural remedies; however, their bioactive components have not been studied. Antioxidant capacity and composition of phenolic compounds of Mentha australis R. Br., Lamiaceae were investigated for the first time. Phenolic compounds were analyzed by HPLC photodiode array detector, liquid chromatography high resolution mass spectrometry, tandem mass spectrometry and nuclear magnetic resonance spectroscopy. Aqueous methanolic extract of the mint exhibited comparable antioxidant capacity to the common spearmint. Major compounds identified in the extract were rosmarinic acid (160.4 ± 0.85 μg mg(-1)purified extract), neoponcirin (145.0 ± 0.42 μg gallic acid equivalent(GAE) mg(-1)), narirutin (30.3 ± 0.02 μg GAE mg(-1)), chlorogenic acid (15.4 ± 0.05 μg mg(-1)) and biochanin A (9.6 ± 0.06 μg GAE mg(-1)), while minor compounds were caffeic acid, apigenin, hesperetin and naringenin. Neoponcirin and biochanin A were identified for the first time in the Mentha genus.

摘要

澳大利亚土生薄荷历来被原住民用作天然疗法；然而，其生物活性成分尚未得到研究。本研究首次对唇形科薄荷属澳大利亚薄荷的抗氧化能力和酚类化合物组成进行了研究。采用高效液相色谱光电二极管阵列检测器、高效液相色谱高分辨质谱、串联质谱和核磁共振波谱对酚类化合物进行了分析。薄荷的水-甲醇提取物表现出与普通留兰香相当的抗氧化能力。提取物中鉴定出的主要化合物为迷迭香酸（160.4 ± 0.85 μg 毫克(-1)纯化提取物）、新蓬苷（145.0 ± 0.42 μg 没食子酸当量（GAE）毫克(-1)）、橙皮苷（30.3 ± 0.02 μg GAE 毫克(-1)）、绿原酸（15.4 ± 0.05 μg 毫克(-1)）和大豆苷元（9.6 ± 0.06 μg GAE 毫克(-1)），而次要化合物为咖啡酸、芹菜素、橙皮苷和柚皮苷。新蓬苷和大豆苷元是首次在薄荷属中被发现的。