Çelik Saliha Esin, Tufan Ayse Nur, Bekdeser Burcu, Özyürek Mustafa, Güçlü Kubilay, Apak Resat
Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar 34320, Istanbul, Turkey.
Istanbul University Application & Research Center for the Measurement of Food Antioxidants, Avcilar 34320, Istanbul, Turkey.
J Chromatogr Sci. 2017 Mar 1;55(3):291-300. doi: 10.1093/chromsci/bmw184.
This study reports the phenolic profile screening of aromatic Lamiaceae species such as marjoram (Origanum majorana L.), lavender (Lavandula officinalis) and pennyroyal (Mentha pulegium L.) using a novel and validated ultra performance liquid chromatography method coupled with DAD diode array detector and tandem mass spectrometry (MS/MS) in negative mode of electrospray ionization. Identification and quantification of phenolics in these plant extracts has been realized within 12 min. This method showed good precision (percentage relative standard deviation; RSD% 0.54-2.72 for intra-day, 1.71-4.64 for inter-day), reproducibility (percentage recovery, REC% 92.0-109.0) and linearity (r = 0.9988-0.9999). Limits of detection ranged from 0.02 to 18.2 ng/mL. The extraction of plants was performed using microwave-assisted extraction technique and 60% (v/v) aqueous methanol solvent medium was selected as suitable solvent because of maximum extraction efficiency. Total antioxidant capacity, total phenolic content and free radical scavenging activity of these plant extracts were tested and the results correlated well among each other. According to the Folin assay, phenolic contents of Origanum majorana L., Mentha pulegium L. and Lavandula officinalis were calculated as 119 ± 3.4, 85.1 ± 2.8 and 57.8 ± 2.1 mg GAE/g dry matter, respectively.
本研究报告了使用一种新型且经过验证的超高效液相色谱法，结合二极管阵列检测器（DAD）和电喷雾电离负离子模式下的串联质谱（MS/MS），对芳香唇形科植物如马郁兰（牛至）、薰衣草和薄荷进行酚类物质谱筛选。在12分钟内实现了对这些植物提取物中酚类物质的鉴定和定量。该方法显示出良好的精密度（相对标准偏差百分比；日内RSD%为0.54 - 2.72，日间为1.71 - 4.64）、重现性（回收率百分比，REC%为92.0 - 109.0）和线性（r = 0.9988 - 0.9999）。检测限范围为0.02至18.2 ng/mL。采用微波辅助萃取技术对植物进行萃取，并选择60%（v/v）的甲醇水溶液作为合适的溶剂，因为其萃取效率最高。对这些植物提取物的总抗氧化能力、总酚含量和自由基清除活性进行了测试，结果相互之间具有良好的相关性。根据福林法测定，牛至、薄荷和薰衣草的酚含量分别计算为119±3.4、85.1±2.8和57.8±2.1 mg GAE/g干物质。
