载脂蛋白B mRNA编辑酶催化多肽样蛋白3B缺失多态性与HIV感染者中乙型肝炎病毒、丙型肝炎病毒、细小病毒B19及弓形虫合并感染的患病率相关。

The APOBEC3B deletion polymorphism is associated with prevalence of hepatitis B virus, hepatitis C virus, Torque Teno virus, and Toxoplasma gondii co-infection among HIV-infected individuals.

作者信息

Prasetyo Afiono Agung, Sariyatun Ratna, Sari Yulia, Haryati Sri, Adnan Zainal Arifin, Kageyama Seiji

机构信息

A-IGIC (A-Infection, Genomic, Immunology & Cancer) Research Group, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia; Center of Biotechnology and Biodiversity Research and Development, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia; Department of Microbiology Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia.

出版信息

J Clin Virol. 2015 Sep;70:67-71. doi: 10.1016/j.jcv.2015.07.009. Epub 2015 Jul 8.

DOI:10.1016/j.jcv.2015.07.009

PMID:26305823

Abstract

BACKGROUND

Data regarding the influence of the APOBEC3B deletion on infectious diseases remain limited and shown discrepancies.

OBJECTIVES

To characterize the APOBEC3B deletion polymorphism status and its association with prevalence of co-infection with blood-borne pathogens in Indonesian HIV-infected individuals.

MATERIALS AND METHODS

A total of 597 HIV-positive blood samples were tested for the hepatitis B virus (HBV), hepatitis C virus (HCV), Torque Teno virus (TTV), GB virus-C (GBV-C), and Toxoplasma gondii. Nucleic acid was extracted from plasma samples and used for the molecular detection of HIV RNA, HBV DNA, HCV RNA, TTV DNA, and GBV-C RNA, whereas HBsAg, anti-HCV, IgM and IgG anti-T. gondii were detected through serological testing. The APOBEC3B deletion polymorphism was genotyped by polymerase chain reaction (PCR).

RESULTS

The deletion genotype was associated with HCV viremia (p<0.001) as well as elevated IgG anti-T. gondii (adjusted OR [aOR]=3.4). The deletion genotype was also associated with decreased levels of HBsAg (aOR=0.03), and anti-HCV (aOR=0.1). D/D was frequently found in HIV-infected individuals with CD4+T cells<14% (aOR=5.8). The intact genotype was associated with a reduced likelihood of a CD4+T cell count<200 cells/μL (aOR=0.2) but a higher prevalence of TTV co-infection (aOR=8.6).

CONCLUSIONS

The APOBEC3B deletion polymorphism was found to be associated with HBV, HCV, TTV, and T. gondii co-infection in Indonesian HIV-infected individuals.

摘要

背景

关于载脂蛋白B mRNA编辑酶催化多肽样3B（APOBEC3B）缺失对传染病影响的数据仍然有限且存在差异。

目的

描述印度尼西亚HIV感染者中APOBEC3B缺失多态性状态及其与血源性病原体合并感染患病率的关联。

材料与方法

共检测597份HIV阳性血液样本中的乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）、扭矩Tenovirus（TTV）、GB病毒C型（GBV-C）和弓形虫。从血浆样本中提取核酸，用于HIV RNA、HBV DNA、HCV RNA、TTV DNA和GBV-C RNA的分子检测，而HBsAg、抗HCV、IgM和IgG抗弓形虫则通过血清学检测。通过聚合酶链反应（PCR）对APOBEC3B缺失多态性进行基因分型。

结果

缺失基因型与HCV病毒血症（p<0.001）以及IgG抗弓形虫升高（校正比值比[aOR]=3.4）相关。缺失基因型还与HBsAg水平降低（aOR=0.03）和抗HCV水平降低（aOR=0.1）相关。D/D基因型在CD4+T细胞<14%的HIV感染者中经常出现（aOR=5.8）。完整基因型与CD4+T细胞计数<200个/μL的可能性降低（aOR=0.2）相关，但TTV合并感染的患病率较高（aOR=8.6）。