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绿色狐尾草(Setaria viridis)的完整叶绿体基因组序列,C4光合作用的一个有前景的模式系统。

Complete chloroplast genome sequence of green foxtail (Setaria viridis), a promising model system for C4 photosynthesis.

作者信息

Wang Shuo, Gao Li-Zhi

机构信息

a Faculty of Environmental Science and Engineering and.

b Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3707-8. doi: 10.3109/19401736.2015.1079867. Epub 2015 Aug 24.

Abstract

The complete chloroplast genome of green foxtail (Setaria viridis), a promising model system for C4 photosynthesis, is first reported in this study. The genome harbors a large single copy (LSC) region of 81 016 bp and a small single copy (SSC) region of 12 456  bp separated by a pair of inverted repeat (IRa and IRb) regions of 22 315 bp. GC content is 38.92%. The proportion of coding sequence is 57.97%, comprising of 111 (19 duplicated in IR regions) unique genes, 71 of which are protein-coding genes, four are rRNA genes, and 36 are tRNA genes. Phylogenetic analysis indicated that S. viridis was clustered with its cultivated species S. italica in the tribe Paniceae of the family Poaceae. This newly determined chloroplast genome will provide valuable genetic resources to assist future studies on C4 photosynthesis in grasses.

摘要

本研究首次报道了绿色狗尾草(Setaria viridis)的完整叶绿体基因组,它是用于C4光合作用研究的一个很有前景的模式系统。该基因组包含一个81016 bp的大单拷贝(LSC)区域和一个12456 bp的小单拷贝(SSC)区域,这两个区域被一对22315 bp的反向重复(IRa和IRb)区域隔开。GC含量为38.92%。编码序列的比例为57.97%,由111个(19个在IR区域重复)独特基因组成,其中71个是蛋白质编码基因,4个是rRNA基因,36个是tRNA基因。系统发育分析表明,绿色狗尾草与它的栽培种粟(Setaria italica)在禾本科黍族中聚类在一起。这个新确定的叶绿体基因组将为未来禾本科C4光合作用的研究提供有价值的遗传资源。

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