Shen Liangying, Qi Zewen, Dai Xiuru, Ai Ye, Chen Jiabao, Chao Yuehui, He Hang, Han Liebao, Xu Lixin
School of Grassland Science, Beijing Forestry University, Beijing, 100083, China.
Institute of Advanced Agricultural Sciences, Peking University, Shandong, 261325, China.
Sci Data. 2025 Apr 3;12(1):571. doi: 10.1038/s41597-025-04827-x.
Zoysiagrass stands out as a crucial native turfgrass due to its exceptional abiotic stress tolerance, extensive adaptability, and high ornamental value. In this study, we generated a high-quality chromosome-level genome assembly of Compadre (COM) zoysiagrass, leveraging PacBio SMRT sequencing and Hi-C scaffolding technologies. The resulting genome assembly (312.42 Mb) is anchored on 20 chromosomes, with a Scaffold N50 of 18.72 Mb. In total, 49,074 genes and 306,768 repeat sequences were annotated in the assembled genome. The first chromosome-scale genome of Zoysia japonica 'Compadre' provides a critical genetic resource for cold-tolerant turfgrass breeding through identifying stress-responsive candidate genes. Additionally, we have successfully established a cell nucleus extraction and library construction protocol tailored for zoysiagrass ATAC-seq technology, and a total of 80 low temperature tolerance candidate genes were preliminarily identified via ATAC-seq and RNA-seq profiling, thereby initiating the exploration of turfgrass epigenomics.
由于其卓越的非生物胁迫耐受性、广泛的适应性和较高的观赏价值，结缕草作为一种关键的本土草坪草脱颖而出。在本研究中，我们利用PacBio SMRT测序和Hi-C支架技术，生成了高质量的Compadre（COM）结缕草染色体水平基因组组装。所得的基因组组装（312.42 Mb）锚定在20条染色体上，支架N50为18.72 Mb。在组装的基因组中总共注释了49,074个基因和306,768个重复序列。日本结缕草“Compadre”的首个染色体规模基因组通过鉴定胁迫响应候选基因，为耐寒草坪草育种提供了关键的遗传资源。此外，我们成功建立了针对结缕草ATAC-seq技术的细胞核提取和文库构建方案，并通过ATAC-seq和RNA-seq分析初步鉴定了总共80个低温耐受性候选基因，从而开启了草坪草表观基因组学的探索。
