Chaves-Moreno Diego, Plumeier Iris, Kahl Silke, Krismer Bernhard, Peschel Andreas, Oxley Andrew P A, Jauregui Ruy, Pieper Dietmar H
Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.
Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology, Eberhard-Karls-University, Geschwister-Scholl-Platz, 72074, Tübingen, Germany.
Environ Microbiol Rep. 2015 Dec;7(6):929-35. doi: 10.1111/1758-2229.12334. Epub 2015 Oct 20.
The cotton rat nose is commonly used as a model for Staphylococcus aureus colonization, as it is both physiologically and anatomically comparable to the human nares and can be easily colonized by this organism. However, while the colonization of the human anterior nares has been extensively studied, the microbial community structure of cotton rat noses has not been reported so far. We describe here the microbial community structure of the cotton rat (Sigmodon hispidus) nose through next-generation sequencing of 16S rRNA gene amplicons covering the V1-V2 region and the analysis of nearly full length 16S rRNA genes of the major phylotypes. Roughly half of the microbial community was composed of two undescribed species of the genus Campylobacter, with phylotypes belonging to the genera Catonella, Acholeplasma, Streptobacillus and Capnocytophaga constituting the predominant community members. Thus, the nasal community of the cotton rat is uniquely composed of several novel bacterial species and may not reflect the complex interactions that occur in human anterior nares. Mammalian airway microbiota may, however, be a rich source of hitherto unknown microbes.
棉鼠的鼻子通常被用作金黄色葡萄球菌定植的模型，因为它在生理和解剖学上与人类鼻腔具有可比性，并且这种生物体很容易在其上定植。然而，虽然对人类前鼻孔的定植进行了广泛研究，但迄今为止尚未报道棉鼠鼻子的微生物群落结构。我们在此通过对覆盖V1-V2区域的16S rRNA基因扩增子进行二代测序以及对主要系统发育型的近乎全长16S rRNA基因进行分析，描述了棉鼠（刚毛囊鼠）鼻子的微生物群落结构。大约一半的微生物群落由弯曲杆菌属的两个未描述物种组成，属于卡顿氏菌属、无胆甾原体属、链杆菌属和二氧化碳嗜纤维菌属的系统发育型构成了主要的群落成员。因此，棉鼠的鼻腔群落独特地由几种新细菌物种组成，可能无法反映人类前鼻孔中发生的复杂相互作用。然而，哺乳动物气道微生物群可能是迄今未知微生物的丰富来源。
