Gelmez Elif, Lehr Konrad, Kershaw Olivia, Frentzel Sarah, Vilchez-Vargas Ramiro, Bank Ute, Link Alexander, Schüler Thomas, Jeron Andreas, Bruder Dunja
Infection Immunology Group, Institute of Medical Microbiology and Hospital Hygiene, Otto-von-Guericke University, 39120 Magdeburg, Germany.
Department of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-Associated Diseases, Otto-von-Guericke University, 39120 Magdeburg, Germany.
Biomedicines. 2022 Aug 5;10(8):1903. doi: 10.3390/biomedicines10081903.
Inflammatory bowel disease (IBD) is a chronic recurrent inflammatory disease with unknown etiology. Dextran sulfate sodium (DSS) induced colitis is a widely used mouse model in IBD research. DSS colitis involves activation of the submucosal immune system and can be used to study IBD-like disease characteristics in acute, chronic, remission and transition phases. Insight into colon inflammatory parameters is needed to understand potentially irreversible adaptations to the chronification of colitis, determining the baseline and impact of further inflammatory episodes. We performed analyses of non-invasive and invasive colitis parameters in acute, chronic and remission phases of the DSS colitis in C57BL/6 mice. Non-invasive colitis parameters poorly reflected inflammatory aspects of colitis in chronic remission phase. We found invasive inflammatory parameters, positively linked to repeated DSS-episodes, such as specific colon weight, inflamed colon area, spleen weight, absolute cell numbers of CD4+ and CD8+ T cells as well as B cells, blood IFN-γ level, colonic chemokines BLC and MDC as well as the prevalence of Turicibacter species in feces. Moreover, microbial Lactobacillus species decreased with chronification of disease. Our data point out indicative parameters of recurrent gut inflammation in context of DSS colitis.
炎症性肠病（IBD）是一种病因不明的慢性复发性炎症性疾病。葡聚糖硫酸钠（DSS）诱导的结肠炎是IBD研究中广泛使用的小鼠模型。DSS结肠炎涉及黏膜下免疫系统的激活，可用于研究急性、慢性、缓解和转变期的IBD样疾病特征。为了了解对结肠炎慢性化的潜在不可逆适应情况，确定进一步炎症发作的基线和影响，需要深入了解结肠炎症参数。我们对C57BL/6小鼠DSS结肠炎的急性、慢性和缓解期的非侵入性和侵入性结肠炎参数进行了分析。非侵入性结肠炎参数在慢性缓解期难以反映结肠炎的炎症方面。我们发现侵入性炎症参数与重复的DSS发作呈正相关，如特定的结肠重量、发炎的结肠面积、脾脏重量、CD4+和CD8+T细胞以及B细胞的绝对细胞数、血液中IFN-γ水平、结肠趋化因子BLC和MDC以及粪便中Turicibacter菌属的患病率。此外，微生物乳酸杆菌属随着疾病的慢性化而减少。我们的数据指出了DSS结肠炎背景下复发性肠道炎症的指示性参数。
