Foster T L, Winans L, Helms S J
Appl Environ Microbiol. 1978 May;35(5):937-44. doi: 10.1128/aem.35.5.937-944.1978.
A Bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples. The organism was isolated on a glucose-mineral salts medium with phosphate deleted. Anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite concentration, increases in turbidity, cell count, and dry-cell weight, and decreases in pH and glucose concentration. The optimum hypophosphite concentration for this isolate was 60 microgram/ml, whereas the optimum phosphate concentration was greater than 1,000 microgram/ml, suggesting that higher concentrations of hypophosphite may be toxic to this isolate. Hypophosphite or phosphite utilization was accompanied by little or no detectable accumulation of phosphate in the medium, and 32P-labeled hypophosphite was incorporated into the cell as organic phosphate. When phosphate was present in the medium, the isolate failed to metabolize phosphite. In the presence of phosphite and hypophosphite, the isolate first utilized phosphite and then hypophosphite.
从卡纳维拉尔角土壤样本中分离出一种能够在厌氧条件下利用亚磷酸盐和次磷酸盐的芽孢杆菌属细菌。该菌株是在不含磷酸盐的葡萄糖-矿物盐培养基上分离得到的。对该分离株进行厌氧培养导致次磷酸盐或亚磷酸盐浓度降低、浊度、细胞计数和干重增加，以及pH值和葡萄糖浓度降低。该分离株的最佳次磷酸盐浓度为60微克/毫升，而最佳磷酸盐浓度大于1000微克/毫升，这表明较高浓度的次磷酸盐可能对该分离株有毒。次磷酸盐或亚磷酸盐的利用伴随着培养基中几乎没有或无法检测到磷酸盐的积累，并且32P标记的次磷酸盐作为有机磷酸盐被整合到细胞中。当培养基中存在磷酸盐时，该分离株无法代谢亚磷酸盐。在亚磷酸盐和次磷酸盐存在的情况下，该分离株首先利用亚磷酸盐，然后利用次磷酸盐。
