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牙周炎促进人牙周膜干细胞的增殖并抑制其分化潜能。

Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells.

作者信息

Zheng Wei, Wang Shi, Wang Jianguo, Jin Fang

机构信息

Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin 300041, P.R. China.

Department of Stomatology, The Third Central Hospital, Tianjin 300041, P.R. China.

出版信息

Int J Mol Med. 2015 Oct;36(4):915-22. doi: 10.3892/ijmm.2015.2314. Epub 2015 Aug 17.

DOI:10.3892/ijmm.2015.2314
PMID:26310866
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4564090/
Abstract

The aim of the present study was to investigate the periodontitis-associated changes in the number, proliferation and differentiation potential of human periodontal ligament stem cells (PDLSCs). Cultures of human periodontal ligament cells (PDLCs) were established from healthy donors and donors with periodontitis. The numbers of stem cell were characterized using flow cytometry. PDLSCs were isolated from the PDLCs by immunomagnetic bead selection. Colony‑forming abilities, osteogenic and adipogenic potential, gene expression of cementoblast phenotype, alkaline phosphatase activity and in vivo differentiation capacities were then evaluated. Periodontitis caused an increase in the proliferation of PDLSCs and a decrease in the commitment to the osteoblast lineage. This is reflected by changes in the expression of osteoblast markers. When transplanted into immunocompromised mice, PDLSCs from the healthy donors exhibited the capacity to produce cementum PDL‑like structures, whereas, the inflammatory PDLSCs transplants predominantly formed connective tissues. In conclusion, the data from the present study suggest that periodontitis affects the proliferation and differentiation potential of human PDLSCs in vitro and in vivo.

摘要

本研究的目的是调查人牙周膜干细胞（PDLSCs）数量、增殖及分化潜能与牙周炎相关的变化。从健康供体和患有牙周炎的供体中建立人牙周膜细胞（PDLCs）培养物。使用流式细胞术对干细胞数量进行表征。通过免疫磁珠分选从PDLCs中分离出PDLSCs。然后评估集落形成能力、成骨和成脂潜能、成牙骨质细胞表型的基因表达、碱性磷酸酶活性及体内分化能力。牙周炎导致PDLSCs增殖增加，而成骨细胞谱系的定向分化减少。这通过成骨细胞标志物表达的变化得以体现。当移植到免疫缺陷小鼠体内时，来自健康供体的PDLSCs表现出产生类牙周膜牙骨质结构的能力，而炎性PDLSCs移植主要形成结缔组织。总之，本研究数据表明，牙周炎在体外和体内均会影响人PDLSCs的增殖和分化潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/7b632a930184/IJMM-36-04-0915-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/1768ca437faa/IJMM-36-04-0915-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/1ff400c2b61a/IJMM-36-04-0915-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/7d3e4b2a4b29/IJMM-36-04-0915-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/bdc7feee0178/IJMM-36-04-0915-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/0cded4462002/IJMM-36-04-0915-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/7b632a930184/IJMM-36-04-0915-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/1768ca437faa/IJMM-36-04-0915-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/1ff400c2b61a/IJMM-36-04-0915-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/7d3e4b2a4b29/IJMM-36-04-0915-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/bdc7feee0178/IJMM-36-04-0915-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/0cded4462002/IJMM-36-04-0915-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3e/4564090/7b632a930184/IJMM-36-04-0915-g05.jpg

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