Department of Stomatology, Yidu Central Hospital, Weifang Medical University, Shandong Province, People's Republic of China.
J Tissue Eng Regen Med. 2014 Mar;8(3):226-32. doi: 10.1002/term.1516. Epub 2012 Jun 15.
Periodontal ligament stem cells (PDLSCs) have great potential for regenerating periodontal ligament tissue, which is involved in attaching teeth to the underlying alveolar bone. Recently, PDLSCs were characterized as having both low immunogenicity and profound immunomodulation abilities. Further, transplanted PDLSCs differentiate into osteoblasts in vivo. In the present study, we investigated the immunological characteristics of osteogenic differentiated PDLSCs. We found that PDLSCs expressed mesenchymal stem cells markers, including STRO-1 and CD146, but were negative for CD14, CD34 and CD45. RT-PCR indicated that NCAM1, MSX1 and S100A4 were expressed in PDLSCs. The cells underwent osteogenic and adipogenic differentiation when cultured in defined medium. Osteogenic differentiated PDLSCs failed to stimulate allogeneic T cell proliferation and suppressed phytohaemagglutinin-triggered T cell proliferation. Indomethacin, an inhibitor of prostaglandin E2 (PGE2) production, restored the T cell proliferation inhibited by osteogenic differentiated PDLSCs. These data confirm that osteogenic differentiated PDLSCs have low immunogenicity and demonstrate that they suppress T cell proliferation in vitro through secretion of PGE2.
牙周膜干细胞（PDLSCs）在再生牙周膜组织方面具有巨大潜力，牙周膜组织参与将牙齿附着在下方牙槽骨上。最近，PDLSCs 的特征是具有低免疫原性和深刻的免疫调节能力。此外，移植的 PDLSCs 可在体内分化为成骨细胞。在本研究中，我们研究了成骨分化的 PDLSCs 的免疫学特性。我们发现 PDLSCs 表达间充质干细胞标志物，包括 STRO-1 和 CD146，但不表达 CD14、CD34 和 CD45。RT-PCR 表明 NCAM1、MSX1 和 S100A4 在 PDLSCs 中表达。当在定义的培养基中培养时，这些细胞经历成骨和脂肪分化。成骨分化的 PDLSCs 未能刺激同种异体 T 细胞增殖，并抑制植物血凝素触发的 T 细胞增殖。前列腺素 E2 (PGE2) 产生的抑制剂吲哚美辛恢复了成骨分化的 PDLSCs 抑制的 T 细胞增殖。这些数据证实成骨分化的 PDLSCs 具有低免疫原性，并表明它们通过分泌 PGE2 在体外抑制 T 细胞增殖。
