高尔基体潴泡内蛋白质阵列的原位结构分析。
In situ structural analysis of Golgi intracisternal protein arrays.
作者信息
Engel Benjamin D, Schaffer Miroslava, Albert Sahradha, Asano Shoh, Plitzko Jürgen M, Baumeister Wolfgang
机构信息
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.
出版信息
Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11264-9. doi: 10.1073/pnas.1515337112. Epub 2015 Aug 26.
We acquired molecular-resolution structures of the Golgi within its native cellular environment. Vitreous Chlamydomonas cells were thinned by cryo-focused ion beam milling and then visualized by cryo-electron tomography. These tomograms revealed structures within the Golgi cisternae that have not been seen before. Narrow trans-Golgi lumina were spanned by asymmetric membrane-associated protein arrays that had ∼6-nm lateral periodicity. Subtomogram averaging showed that the arrays may determine the narrow central spacing of the trans-Golgi cisternae through zipper-like interactions, thereby forcing cargo to the trans-Golgi periphery. Additionally, we observed dense granular aggregates within cisternae and intracisternal filament bundles associated with trans-Golgi buds. These native in situ structures provide new molecular insights into Golgi architecture and function.
我们在其天然细胞环境中获得了高尔基体的分子分辨率结构。通过冷冻聚焦离子束研磨使玻璃态衣藻细胞变薄，然后通过冷冻电子断层扫描进行观察。这些断层扫描揭示了高尔基体潴泡内以前未见的结构。狭窄的反式高尔基体腔由具有约6纳米横向周期性的不对称膜相关蛋白阵列跨越。亚断层平均显示，这些阵列可能通过拉链样相互作用决定反式高尔基体潴泡的狭窄中央间距，从而将货物推向反式高尔基体周边。此外，我们在潴泡内观察到致密的颗粒聚集体以及与反式高尔基体芽相关的潴泡内丝状束。这些天然原位结构为高尔基体的结构和功能提供了新的分子见解。
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