Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
Bioresour Technol. 2015 Dec;197:1-6. doi: 10.1016/j.biortech.2015.08.037. Epub 2015 Aug 21.
The cell immobilization potential of a novel xylan based disulfide-crosslinked hydrogel matrix reinforced with cellulose nanocrystals was studied with continuous cultivation of Propionibacterium acidipropionici using various dilution rates. The cells were immobilized to hydrogel beads suspended freely in the fermentation broth or else packed into a column connected to a stirred tank reactor. The maximum propionic acid productivity for the combined stirred tank and column was 0.88gL(-1)h(-1) and the maximum productivity for the column was determined to be 1.39gL(-1)h(-1). The maximum propionic acid titer for the combined system was 13.9gL(-1) with a dilution rate of 0.06h(-1). Dry cell density of 99.7gL(-1) was obtained within the column packed with hydrogel beads and productivity of 1.02gL(-1)h(-1) was maintained in the column even with the high circulation rate of 3.37h(-1).
新型木聚糖基二硫键交联水凝胶基质的细胞固定化潜力，用丙酸杆菌连续培养不同稀释率。细胞固定在悬浮在发酵液中的水凝胶珠中，或者装在与搅拌釜式反应器相连的柱中。搅拌釜和柱的组合最高丙酸产率为 0.88gL(-1)h(-1)，柱的最高产率为 1.39gL(-1)h(-1)。组合系统的最大丙酸浓度为 13.9gL(-1)，稀释率为 0.06h(-1)。用水凝胶珠填充的柱内获得了 99.7gL(-1)的干细胞密度，即使在 3.37h(-1)的高循环速率下，柱内的产率仍保持在 1.02gL(-1)h(-1)。
