Institute of Biological Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2012 May;112:248-53. doi: 10.1016/j.biortech.2012.01.055. Epub 2012 Feb 2.
Propionic acid is an important short-chain fatty acid with many applications, but its large-scale bioproduction was hindered by the low productivity. An adapted acid-tolerant Propionibacterium acidipropionici CGMCC 1.2230 strain was selected to produce propionic acid with a relatively high productivity (0.29 g/(Lh)) in the free-cell fermentation. Further immobilized-cell fermentation in fibrous-bed bioreactor (FBB) supported high-level repeated batch fermentations with a high productivity of 0.96 g/(Lh). The FBB also presents the potential to increase final propionic acid concentration by using glucose feeding strategy. The propionic acid concentration was increased to 51.2g/L in the fed-batch fermentation with the productivity of 0.71 g/(Lh). By adopting the above strategies, sugarcane bagasse hydrolysate could support the production of propionic acid with high productivity in the repeat-batch and fed-batch fermentations. The present work would pave one road to the accomplishment of large-scale bioproduction of propionic acid from renewable resources.
丙酸是一种重要的短链脂肪酸，具有多种应用，但由于生产力低，其大规模生物生产受到阻碍。选择了适应性耐酸丙酸杆菌 CGMCC 1.2230 菌株，在无细胞发酵中以相对较高的生产力（0.29 g/（Lh））生产丙酸。进一步在纤维床生物反应器（FBB）中进行固定化细胞发酵，支持高水平的重复分批发酵，生产力高达 0.96 g/（Lh）。FBB 还具有通过葡萄糖补料策略提高最终丙酸浓度的潜力。在分批补料发酵中，丙酸浓度提高到 51.2g/L，生产力为 0.71 g/（Lh）。通过采用上述策略，甘蔗渣水解物可以支持在重复分批和分批补料发酵中以高生产力生产丙酸。本工作为利用可再生资源大规模生物生产丙酸铺平了道路。
