Liang Xian, Ren Yong-xiang, Yang Lei, Zhao Si-qi, Xia Zhi-hong
Huan Jing Ke Xue. 2015 May;36(5):1749-56.
Traditional process of autotrophic nitrification-anaerobic denitrification usually has problems of long procedure and low efficiency. To overcome these problems, a heterotrophic nitrification-aerobic denitrification bacterium YL was isolated from a domesticated mature SBR reactor with efficient simultaneous nitrification and denitrification ability, and was identified as Pseudomonas aeruginosa YL. Meanwhile, the characteristics of the nitrogen removal of strain YL was investigated through single-factor experiments and an orthogonal experiment. The results showed that the preferred conditions were: succinate as the carbon source, C/N ratio of 10, pH of 7.0, temperature of 30°C, and the shaking speed of 160-200 r · min(-1), while the removal rate of ammonia oxidation was 5. 05 mg · (g · h)(-1), the transformation rate of TOC was 45.95 mg · (g · h)(-1), and the removal rates of nitrogen and TOC were 100% and 90.8%, respectively. Nitrite, nitrate and hydroxylamine could also be metabolized by strain YL, and the removal rates were 92.7%, 93.6% and 94.8%, respectively. The most important influencing factor on aerobic denitrification of strain YL was C/N ratio. Under the optimal conditions (C/N = 10, T = 30°C , r = 200 r · min(-1), pH = 7), the removal rates of nitrate and total nitrogen were 94.6% and 76.3%, respectively. Hence, strain YL could remove nitrogen by heterotrophic nitrification-aerobic denitrification independently, quickly, and effectively.
传统的自养硝化-厌氧反硝化工艺通常存在流程长、效率低的问题。为克服这些问题，从具有高效同步硝化反硝化能力的驯化成熟SBR反应器中分离出一株异养硝化-好氧反硝化细菌YL，并鉴定为铜绿假单胞菌YL。同时，通过单因素实验和正交实验研究了菌株YL的脱氮特性。结果表明，最佳条件为：以琥珀酸为碳源，碳氮比为10，pH为7.0，温度为30℃，摇床转速为160-200 r·min(-1)，此时氨氧化去除率为5.05 mg·(g·h)(-1)，总有机碳转化率为45.95 mg·(g·h)(-1)，氮和总有机碳的去除率分别为100%和90.8%。亚硝酸盐、硝酸盐和羟胺也能被菌株YL代谢，去除率分别为92.7%、93.6%和94.8%。对菌株YL好氧反硝化影响最重要的因素是碳氮比。在最佳条件下（碳氮比=10，温度=30℃，转速=200 r·min(-1)，pH=7），硝酸盐和总氮的去除率分别为94.6%和76.3%。因此，菌株YL能够通过异养硝化-好氧反硝化独立、快速且有效地去除氮。
