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[菌株sari01的分离及其异养硝化-好氧反硝化特性]

[Isolation of sp. sari01 and Its Heterotrophic Nitrification-Aerobic Denitrification Characteristics].

作者信息

Yan Wei-Zhi, Hao Jian, Sun Jun-Song, Shi Ji-Ping

机构信息

College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.

出版信息

Huan Jing Ke Xue. 2016 Jul 8;37(7):2673-2680. doi: 10.13227/j.hjkx.2016.07.034.

DOI:10.13227/j.hjkx.2016.07.034
PMID:29964478
Abstract

The genus of belongs to the family of , and some strains of sp. have the function of degrading chemical pollutants in the environment. A sp. strain was isolated from activated sludge through enrichment cultured with heterotrophic nitrification medium. This strain was named sp. sari01. Single-factor and response surface methodology experiments results showed that efficient heterotrophic nitrification of strain sari01 occurred with sodium citrate as the carbon source, at pH of 7.0-7.5, temperature of 30℃, C/N ratio of 15, inoculation volume of 7.5% and loading volume of 50 mL, while the removal rate of nitrogen was 99.9%, of which 33.7% was converted to gaseous product and escaped to air, and the residual nitrogen was fixed in cell biomass. Using nitrite and nitrate as the sole nitrogen source, the nitrogen degradation ratios were 98.4% and 65.2%, respectively. Hence, strain sari01 could remove nitrogen by heterotrophic nitrification-aerobic denitrification independently, quickly and effectively, which demonstrated that strain sari01 has the potential to be used in wastewater treatment.

摘要

[属名]属于[科名]科,并且[属名]属的一些菌株具有降解环境中化学污染物的功能。通过用异养硝化培养基进行富集培养,从活性污泥中分离出一株[属名]属菌株。该菌株被命名为[属名] sari01。单因素和响应面法实验结果表明,以柠檬酸钠为碳源,在pH为7.0 - 7.5、温度为30℃、碳氮比为15、接种量为7.5%和装液量为50 mL的条件下,菌株sari01发生高效异养硝化作用,同时氮去除率为99.9%,其中33.7%转化为气态产物逸出到空气中,剩余氮固定在细胞生物质中。以亚硝酸盐和硝酸盐作为唯一氮源时,氮降解率分别为98.4%和65.2%。因此,菌株sari01能够通过异养硝化 - 好氧反硝化独立、快速且有效地去除氮,这表明菌株sari01具有用于废水处理的潜力。

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