[B（A）血型的鉴定及B（A）血型患者的输血]
[Identification of B (A) Blood Group and Blood Transfusion for patients with B (A) Blood Group].
作者信息
Wang Chao, Li Su-Ping, Wu Xue-Zhong, Li Min, Xing Xin, Wang Lun-Shan, Lu Rong
机构信息
Anhui Provincial Blood Center, Department of Blood Transfusion, Hefei Blood Center, Hefei 230031, Anhui Province, China.
Department of Laboratorial Examination, The Chinese PLA 105 Hospital, Hefei 230031, Anhui Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):1147-51. doi: 10.7534/j.issn.1009-2137.2015.04.046.
OBJECTIVE
To investigate the serological and molecular biological identification of B(A) blood group and its reasonable method of blood transfusion for patient with B(A) blood group.
METHODS
The blood group of patient was detected by serological method, at the some time, the genotype of patient was detected by using the ABO-TYPE Variant kit and sequence analysis of 6 and 7 exons in ABO gene; the washed O red blood cells were used to cross matching blood of difficultly matching blood by the three step analysis method.
RESULTS
The A weak and B strong agglutination were found in positive type, and A1C(3+), BC(-) were observed in negative type; the molecular biological identification showed B(A)04, 640 A > G; the matching blood main side of washed O red blood cells displayed no agglutination.
CONCLUSION
The identification and analysis of rare blood or subtype should be very careful; if necessary, the molecular biological detection should carried out; the blood transfusion for patient with rate blood group or subtype should be safe, correct and reasonable.
目的
探讨B（A）血型的血清学及分子生物学鉴定方法及其对B（A）血型患者合理的输血方法。
方法
采用血清学方法检测患者血型，同时应用ABO-TYPE Variant试剂盒及对ABO基因第6、7外显子进行序列分析检测患者基因型；采用三步分析法用洗涤O型红细胞对疑难配血患者血液进行交叉配血。
结果
阳性定型时出现A弱、B强凝集，阴性定型时观察到A1C（3+）、BC（-）；分子生物学鉴定显示为B（A）04，640 A＞G；洗涤O型红细胞主侧配血无凝集。
结论
对稀有血型或亚型的鉴定与分析应格外谨慎；必要时应进行分子生物学检测；对稀有血型或亚型患者输血应安全、正确、合理。
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