Nikolić Tatjana V, Purać Jelena, Orčić Snežana, Kojić Danijela, Vujanović Dragana, Stanimirović Zoran, Gržetić Ivan, Ilijević Konstantin, Šikoparija Branko, Blagojević Duško P
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia.
Faculty of Pharmacy, University of Belgrade, Belgrade, Republic of Serbia.
Arch Insect Biochem Physiol. 2015 Dec;90(4):181-94. doi: 10.1002/arch.21253. Epub 2015 Aug 28.
Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajača, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.
了解蜜蜂的细胞应激反应将对其保护工作做出重大贡献。本研究的目的是分析抗氧化酶超氧化物歧化酶和过氧化氢酶在蜜蜂体内的反应，这些反应与不同栖息地中有毒金属的存在有关。选择了三个地点：（i）戈利贾山上的图诺沃，作为对照区域，没有工业活动和大规模人类影响；（ii）作为城市区域的贝尔格莱德；（iii）作为采矿和工业区的扎查察。我们的结果表明，蜜蜂全身的铅（Pb）浓度因栖息地而异，但在受调查的工业区采集的蜜蜂体内，铅含量有非常显著的增加。来自城市和工业区的蜜蜂中，Sod1和Cat基因的表达均有所增加，这表明它们对增加的氧化应激具有适应性。然而，尽管基因表达增加，但工业区蜜蜂体内过氧化氢酶的酶活性较低，这表明铅对过氧化氢酶有抑制作用。
