Roberts B, Hirst R
Department of Biomedical and Tropical Veterinary Sciences, James Cook University of North Queensland, Townsville, Australia.
J Med Microbiol. 1996 Nov;45(5):383-7. doi: 10.1099/00222615-45-5-383.
Previous investigations have demonstrated the presence of both superoxide dismutase and catalase enzymes in several intracellular pathogens, including a number of mycobacterial species. These enzymes are believed to be involved in the protection of the pathogen from the bactericidal products of oxidative metabolism. Superoxide dismutase and catalase were identified in crude extracts of Mycobacterium ulcerans by polyacrylamide gel electrophoresis. Inhibition experiments showed that the superoxide dismutase probably contained manganese as the metal cofactor. Other mycobacterial species examined for comparison produced bands of superoxide dismutase activity with a different mobility to that of M. ulcerans, suggesting possible structural differences between the enzymes.
先前的研究表明，包括多种分枝杆菌在内的几种细胞内病原体中都存在超氧化物歧化酶和过氧化氢酶。据信这些酶参与保护病原体免受氧化代谢杀菌产物的侵害。通过聚丙烯酰胺凝胶电泳在溃疡分枝杆菌的粗提物中鉴定出了超氧化物歧化酶和过氧化氢酶。抑制实验表明，超氧化物歧化酶可能含有锰作为金属辅因子。为作比较而检测的其他分枝杆菌物种产生的超氧化物歧化酶活性条带与溃疡分枝杆菌的条带迁移率不同，这表明这些酶可能存在结构差异。
