Lan Bin, Zhang Jian, Lu Dong, Li Wenqin
Department of Gastroenterological Surgery, The First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd., Fuzhou 350005, Fujian, China.
Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, 29 Xinquan Rd., Fuzhou 350001, Fujian, China.
Immunobiology. 2016 Jan;221(1):1-5. doi: 10.1016/j.imbio.2015.08.010. Epub 2015 Aug 18.
The therapy of colon cancer (Cca) is poor currently. The abnormality of immune function is recognized in cancer-bearing hosts. This study aims to inhibit Cca growth with the in vivo induction of Cca-specific CD8(+) CD69(+) T cell polarization. In this study, a mouse tumor model was created with a mouse colon cancer (Cca) cell line. The Cca-bearing mice were immunized with Cca extracts and alum (using as an adjuvant). The CD8(+) CD69(+) cell polarization status was analyzed by flow cytometry and enzyme-linked immunosorbent assay. The results showed that a Cca antigen-specific CD8(+) CD69(+) cells were induced in the Cca-bearing mice. The Cca growth was efficiently inhibited by the Cca-specific CD8(+) CD69(+) cell response. The Cca-specific CD8(+) CD69(+) cells were detected in the Cca mass and spleen, which had the ability to induce Cca cell apoptosis. In conclusion, the Cca-specific CD8(+) CD69(+) cell polarization can be induced in Cca-bearing mice, which can efficiently inhibit the Cca growth.
目前结肠癌（Cca）的治疗效果不佳。癌症宿主中存在免疫功能异常。本研究旨在通过体内诱导Cca特异性CD8(+) CD69(+) T细胞极化来抑制Cca生长。在本研究中，用小鼠结肠癌细胞系建立了小鼠肿瘤模型。荷Cca小鼠用Cca提取物和明矾（用作佐剂）进行免疫。通过流式细胞术和酶联免疫吸附测定分析CD8(+) CD69(+)细胞极化状态。结果显示，荷Cca小鼠中诱导出了Cca抗原特异性CD8(+) CD69(+)细胞。Cca特异性CD8(+) CD69(+)细胞应答有效抑制了Cca生长。在Cca肿块和脾脏中检测到Cca特异性CD8(+) CD69(+)细胞，它们具有诱导Cca细胞凋亡的能力。总之，荷Cca小鼠中可诱导出Cca特异性CD8(+) CD69(+)细胞极化，其可有效抑制Cca生长。
