体外评估用于定量釉质脱矿的增强反射荧光成像（FIRE）
Evaluation of Fluorescence Imaging with Reflectance Enhancement (FIRE) for Quantifying Enamel Demineralization In vitro.
作者信息
Xiao Qiang, Tu Rui, He Tao, Yin Wei, Li Xue, Hu Deyu, Zhang Ximu
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
Caries Res. 2015;49(5):531-9. doi: 10.1159/000365298. Epub 2015 Aug 28.
OBJECTIVES
To assess the ability of fluorescence imaging with reflectance enhancement (FIRE) to quantify early enamel demineralization in vitro.
METHODS
A total of 30 bovine enamel specimens were immersed in demineralizing solution to produce artificial caries. Specimens were examined by FIRE, quantitative light-induced fluorescence (QLF), and polarized light microscopy (PLM) at baseline and after 0.5, 1, 2, 4, 8, 24, 48 and 72 h of immersion. Fluorescence loss measured by FIRE and QLF was compared with lesion depth measurements by PLM.
RESULTS
Over all time points, measurement of fluorescence loss by FIRE correlated well with the measurement of x0394;F by QLF and of x0394;Q by QLF. Both FIRE and QLF techniques showed significant correlation with PLM.
CONCLUSION
FIRE technology may be useful for quantifying enamel demineralization in vitro.
目的
评估反射增强荧光成像（FIRE）在体外定量早期釉质脱矿的能力。
方法
总共30个牛牙釉质标本浸入脱矿溶液中以产生人工龋。在基线以及浸泡0.5、1、2、4、8、24、48和72小时后，通过FIRE、定量光诱导荧光（QLF）和偏振光显微镜（PLM）对标本进行检查。将FIRE和QLF测量的荧光损失与PLM测量的病变深度进行比较。
结果
在所有时间点，FIRE测量的荧光损失与QLF测量的ΔF以及QLF测量的ΔQ相关性良好。FIRE和QLF技术均与PLM显示出显著相关性。
结论
FIRE技术可能有助于体外定量釉质脱矿。
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