Ando M, van Der Veen M H, Schemehorn B R, Stookey G K
Department of Preventive and Community Dentistry, Oral Health Research Institute, Indiana University School of Dentistry, Indianapolis, IN 46202-2876, USA.
Caries Res. 2001 Nov-Dec;35(6):464-70. doi: 10.1159/000047491.
Caries is a disease that affects both deciduous and permanent dentitions. Caries progresses more rapidly in deciduous enamel than in permanent enamel. Therefore, new caries diagnostic methods need to be tested on the deciduous teeth as well. Quantitative laser-induced fluorescence (QLF I) as well as the quantitative light-induced fluorescence (QLF II) seem promising for the quantification of mineral loss from dental caries but have only been tested on the permanent dentition. The objective of this study was to determine and compare the ability of QLF I and QLF II to quantify mineral loss from carious lesions in both deciduous and permanent teeth. Thirty sound deciduous and 30 sound permanent teeth were cleaned and divided into three groups each containing 10 deciduous and 10 permanent teeth. Windows on the buccal or labial enamel surfaces were demineralized for 48, 72, or 96 h. Images of demineralized enamel were captured using QLF I and QLF II. The images were analyzed to determine the mean change in fluorescence radiance (Delta F, %). The teeth were then sectioned for assessment of lesion depth (microm) and integrated mineral loss (IML, vol% x microm) using transverse microradiography (TMR), as the 'gold standard' for lesion analysis. The results indicated a good correlation for Delta F between QLF I and QLF II in both deciduous (r = 0.96) and permanent teeth (r = 0.98). There was a good correlation between Delta F and TMR (lesion depth and IML) in deciduous teeth (r = 0.76 and 0.84 with QLF I, r = 0.81 and 0.88 with QLF II). In permanent teeth, the correlation between Delta F and TMR (lesion depth and IML) was lower than in deciduous teeth (r = 0.07 and 0.53 with QLF I, r = 0.15 and 0.62 with QLF II). From these results it can be concluded that either QLF method is capable of quantifying mineral loss in early carious lesions in deciduous teeth. Moreover, under the conditions of this study, the use of either QLF method to quantify mineral loss in early carious lesions in deciduous teeth is slightly more accurate than in permanent teeth.
龋齿是一种影响乳牙和恒牙列的疾病。龋齿在乳牙釉质中的进展比在恒牙釉质中更快。因此，新的龋齿诊断方法也需要在乳牙上进行测试。定量激光诱导荧光（QLF I）以及定量光诱导荧光（QLF II）在量化龋齿导致的矿物质流失方面似乎很有前景，但仅在恒牙列上进行过测试。本研究的目的是确定并比较QLF I和QLF II量化乳牙和恒牙龋齿病变中矿物质流失的能力。30颗健康乳牙和30颗健康恒牙被清洁后分为三组，每组包含10颗乳牙和10颗恒牙。颊侧或唇侧釉质表面的窗口进行脱矿处理48、72或96小时。使用QLF I和QLF II拍摄脱矿釉质的图像。对图像进行分析以确定荧光辐射率的平均变化（ΔF，%）。然后将牙齿切片，使用横向显微放射照相术（TMR）评估病变深度（微米）和累积矿物质流失（IML，体积%×微米），作为病变分析的“金标准”。结果表明，QLF I和QLF II在乳牙（r = 0.96）和恒牙（r = 0.98）中的ΔF之间具有良好的相关性。乳牙中ΔF与TMR（病变深度和IML）之间具有良好的相关性（QLF I时r = 0.76和0.84；QLF II时r = 0.81和0.88）。在恒牙中，ΔF与TMR（病变深度和IML）之间的相关性低于乳牙（QLF I时r = 0.07和0.53；QLF II时r = 0.15和0.62）。从这些结果可以得出结论，两种QLF方法都能够量化乳牙早期龋齿病变中的矿物质流失。此外，在本研究条件下，使用任何一种QLF方法量化乳牙早期龋齿病变中的矿物质流失都比恒牙稍准确。
