Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, CF14 4XN, UK.
Int J Oncol. 2015 Oct;47(4):1429-39. doi: 10.3892/ijo.2015.3121. Epub 2015 Aug 13.
Metastasis suppressor 1 (MTSS1) is a potential metastasis suppressor gene involved in the regulation of cytoskeleton dynamics and subsequently in cell motility. MTSS1 expression is frequently reduced in a variety of cancer cells and tissues and this loss may account for increased invasive traits in cancer cells. The present study aimed to assess the role of MTSS1 in epithelial ovarian cancer (EOC) cells. Expression of MTSS1 in human ovarian cells was assessed at both the mRNA and protein levels using reverse transcription-PCR (RT-PCR) and immunohistochemistry, respectively. Full-length MTSS1 cDNA expression vector was used to generate MTSS1 overexpressing cells. The effect of MTSS1 overexpression on cellular functions was examined in EOC cells using a variety of in vitro assays. MTSS1 expression was observed both in ovarian cancer tissues and EOC cells. Over-expression of MTSS1 protein reduced the growth, invasion, adhesion and migration of EOC cell lines in vitro. The present study revealed that MTSS1 plays an essential inhibitory role in the development and progression of ovarian cancers. MTSS1 overexpression is intimately related to migration and metastasis, suggesting that MTSS1 is a potential prognostic marker and therapeutic molecular target in human ovarian cancer.
转移抑制因子 1（MTSS1）是一种潜在的转移抑制基因，参与细胞骨架动力学的调节，进而影响细胞的迁移能力。MTSS1 在多种癌细胞和组织中表达下调，这种下调可能导致癌细胞侵袭性增加。本研究旨在评估 MTSS1 在卵巢上皮性癌（EOC）细胞中的作用。采用逆转录-PCR（RT-PCR）和免疫组织化学分别检测 MTSS1 在人卵巢细胞中的 mRNA 和蛋白表达水平。利用全长 MTSS1 cDNA 表达载体生成 MTSS1 过表达细胞。通过多种体外实验研究 MTSS1 过表达对 EOC 细胞的细胞功能的影响。在卵巢癌组织和 EOC 细胞中均观察到 MTSS1 表达。MTSS1 蛋白过表达可降低 EOC 细胞系的体外生长、侵袭、黏附和迁移能力。本研究表明，MTSS1 在卵巢癌的发生发展中发挥重要的抑制作用。MTSS1 过表达与迁移和转移密切相关，提示 MTSS1 可能是人类卵巢癌的一个潜在预后标志物和治疗性分子靶标。
