Costa Felipe Lobato da Silva, Teixeira Renan Kleber Costa, Yamaki Vitor Nagai, Valente André Lopes, Silva Andressa Miléo Ferraioli, Brito Marcus Vinicius Henriques, Percário Sandro
Laboratory for Experimental Surgery, Department of Experimental Surgery, Pará State University, Belém, Pará, Brazil.
Laboratory for Experimental Surgery, Department of Experimental Surgery, Pará State University, Belém, Pará, Brazil.
J Surg Res. 2016 Jan;200(1):105-9. doi: 10.1016/j.jss.2015.07.031. Epub 2015 Jul 22.
Remote ischemic conditioning (RIC) is the most promising surgical approach to mitigate ischemia and reperfusion (IR) injury. It consists in performing brief cycles of IR in tissues other than those exposed to ischemia. The underlying mechanisms of the induced protection are barely understood, so we evaluated if RIC works enhancing the antioxidant defense of the liver and kidney before IR injury.
Twenty-one Wistar rats were assigned into three groups as follows: sham, same surgical procedure as in the remaining groups was performed, but no RIC was carried out. RIC 10, RIC was performed, and no abdominal organ ischemia was induced. After 10 min of the end of the RIC protocol, the liver and kidney were harvested. RIC 60, similar procedure as performed in RIC 10, but the liver and the kidney were harvested 60 min. RIC consisted of three cycles of 5-min left hind limb ischemia followed by 5-min left hind limb perfusion, lasting 30 min in total. Samples were used to measure tissue total antioxidant capacity.
RIC protocol increased both liver (1.064 ± 0.26 mM/L) and kidney (1.310 ± 0.17 mM/L) antioxidant capacity after 10 min when compared with sham (liver, 0.759 ± 0.10 mM/L and kidney, 1.08 ± 0.15 mM/L). Sixty minutes after the RIC protocol, no enhancement on liver (0.687 ± 0.13 mM/L) or kidney (1.09 ± 0.15 mM/L) antioxidant capacity was detected.
RIC works through temporary and short-term enhancement of liver and kidney cells antioxidant defenses to avoid the deleterious consequences of a future IR injury.
远程缺血预处理（RIC）是减轻缺血再灌注（IR）损伤最具前景的外科手术方法。它包括在未暴露于缺血的组织中进行短暂的IR循环。诱导性保护的潜在机制尚不清楚，因此我们评估了RIC是否通过增强肝脏和肾脏在IR损伤前的抗氧化防御作用来发挥作用。
将21只Wistar大鼠分为三组：假手术组，进行与其他组相同的手术操作，但不进行RIC；RIC 10组，进行RIC，不诱导腹部器官缺血。在RIC方案结束10分钟后，采集肝脏和肾脏；RIC 60组，操作与RIC 10组相似，但在60分钟后采集肝脏和肾脏。RIC包括三个5分钟的左后肢缺血循环，随后是5分钟的左后肢灌注，共持续30分钟。采集样本以测量组织总抗氧化能力。
与假手术组（肝脏，0.759±0.10 mM/L；肾脏，1.08±0.15 mM/L）相比，RIC方案在10分钟后提高了肝脏（1.064±0.26 mM/L）和肾脏（1.310±0.17 mM/L）的抗氧化能力。在RIC方案60分钟后，未检测到肝脏（0.687±0.13 mM/L）或肾脏（1.09±0.15 mM/L）抗氧化能力的增强。
RIC通过暂时和短期增强肝脏和肾脏细胞的抗氧化防御作用来避免未来IR损伤的有害后果。
