Sousa Maria Lígia, Figueiredo Francisco, Pinheiro Catarina, Silva Ana, Malhão Fernanda, Rocha Maria João, Rocha Eduardo, Urbatzka Ralph
CIIMAR - Interdisciplinary Center of Marine and Environmental ResearchCIMAR Associated Laboratory, U. Porto - University of Porto, Rua dos Bragas 289, 4050-123 Porto, PortugalICBAS - Institute of Biomedical Sciences Abel SalazarUPorto - University of Porto, Rua de Jorge Viterbo Ferreira n° 228, 4050-313 Porto, PortugalISCS-N - Superior Institute of Health Sciences-NorthCESPU - Cooperative Higher Education, Polytechnic and University, Rua Central da Gandra 1317, 4585-116 Gandra, Portugal CIIMAR - Interdisciplinary Center of Marine and Environmental ResearchCIMAR Associated Laboratory, U. Porto - University of Porto, Rua dos Bragas 289, 4050-123 Porto, PortugalICBAS - Institute of Biomedical Sciences Abel SalazarUPorto - University of Porto, Rua de Jorge Viterbo Ferreira n° 228, 4050-313 Porto, PortugalISCS-N - Superior Institute of Health Sciences-NorthCESPU - Cooperative Higher Education, Polytechnic and University, Rua Central da Gandra 1317, 4585-116 Gandra, Portugal.
CIIMAR - Interdisciplinary Center of Marine and Environmental ResearchCIMAR Associated Laboratory, U. Porto - University of Porto, Rua dos Bragas 289, 4050-123 Porto, PortugalICBAS - Institute of Biomedical Sciences Abel SalazarUPorto - University of Porto, Rua de Jorge Viterbo Ferreira n° 228, 4050-313 Porto, PortugalISCS-N - Superior Institute of Health Sciences-NorthCESPU - Cooperative Higher Education, Polytechnic and University, Rua Central da Gandra 1317, 4585-116 Gandra, Portugal.
Reproduction. 2015 Nov;150(5):429-36. doi: 10.1530/REP-15-0271. Epub 2015 Aug 27.
Oogenesis in zebrafish (Danio rerio) is controlled by the hypothalamus-pituitary-gonadal axis and reproductive hormones. In addition, an interference of stress hormones is known with reproductive biology. In the presented work, we aimed to explore the hypothesis that cortisol (Cort) and ACTH may affect early oogenesis in zebrafish, given the presence of the specific receptors for glucocorticoids and ACTH in the zebrafish ovary. Follicles at stages I and II were exposed in vitro to 1 μM Cort and ACTH for 48 h, then ultrastructural and molecular effects were analyzed. The comet assay demonstrated increased tail moments for Cort and ACTH treatment indicative of DNA damage. The mRNA expression of apoptotic genes (bax, bcl-2) was not altered by both treatments, but Cort increased significantly the expression of the ACTH receptor (mc2r). Cort stimulated the presence of the endoplasmic reticulum, predominantly at stage II, while ACTH induced a strong vacuolization. Viability of oocytes was not affected by both treatments and fluorescent staining (monodansylcadaverine/acridine orange) indicated a reduced quantity of autophagosomes for ACTH, and lower presence of nucleic acids in ooplasm for Cort and ACTH. Concluding, different responses were observed for stress hormones on early stages of zebrafish oocytes, which suggest a role for both hormones in the stress-mediated adverse effects on female gametogenesis.
斑马鱼（Danio rerio）的卵子发生受下丘脑 - 垂体 - 性腺轴及生殖激素的调控。此外，已知应激激素会干扰生殖生物学过程。在本研究中，鉴于斑马鱼卵巢中存在糖皮质激素和促肾上腺皮质激素（ACTH）的特异性受体，我们旨在探究皮质醇（Cort）和ACTH可能影响斑马鱼早期卵子发生的假说。将I期和II期卵泡在体外暴露于1 μM的Cort和ACTH中48小时，然后分析其超微结构和分子效应。彗星试验表明，Cort和ACTH处理组的尾矩增加，表明存在DNA损伤。两种处理均未改变凋亡基因（bax、bcl - 2）的mRNA表达，但Cort显著增加了ACTH受体（mc2r）的表达。Cort主要在II期刺激内质网的出现，而ACTH则诱导强烈的空泡化。两种处理均未影响卵母细胞的活力，荧光染色（单丹磺酰尸胺/吖啶橙）表明，ACTH处理组的自噬体数量减少，Cort和ACTH处理组的卵质中核酸含量降低。总之，在斑马鱼卵母细胞早期阶段观察到应激激素的不同反应，这表明这两种激素在应激介导的对雌性配子发生的不利影响中发挥作用。
