Molania Ramyar, Mahjoubi Frouzandeh, Mirzaei Rezvan, Khatami Saeed-Reza, Mahjoubi Bahar
Division of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Boulevard, Tehran-Karaj Highway, 17th Km, P.O. Box 14965-161, Tehran, Iran ; Department of Genetics, Faculty of Science, Shahid Chamran University, Ahwaz, Iran.
Division of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Boulevard, Tehran-Karaj Highway, 17th Km, P.O. Box 14965-161, Tehran, Iran.
J Biomark. 2014;2014:272683. doi: 10.1155/2014/272683. Epub 2014 Mar 9.
Colorectal cancer (CRC) is the third common carcinoma with a high rate of mortality worldwide and several studies have investigated some molecular and clinicopathological markers for diagnosis and prognosis of its malignant phenotypes. The aim of this study is to evaluate expression frequency of PAGE4, SCP-1, and SPANXA/D cancer testis antigen (CTA) genes as well as some clinical risk markers to predict liver metastasis of colorectal cancer patients. The expression frequency of PAGE4, SCP-1, and SPANXA/D cancer/testis antigen (CTA) genes was obtained using reverse transcription polymerase chain reaction (RT-PCR) assay in 90 colorectal tumor samples including both negative and positive liver metastasis tumors. Statistical analysis was performed to assess the association of three studied genes and clinical risk factors with CRC liver metastasis. The frequency of PAGE4 and SCP-1 genes expression was significantly higher in the primary tumours with liver metastasis when statistically compared with primary tumors with no liver metastasis (P < 0.05). Among all clinical risk factors studied, the lymph node metastasis and the depth of invasion were statistically correlated with liver metastasis of CRC patients. In addition, using multiple logistic regression, we constructed a model based on PAGE4 and lymph node metastasis to predict liver metastasis of CRC.
结直肠癌（CRC）是全球第三大常见癌症，死亡率很高，多项研究已对其恶性表型的一些分子和临床病理标志物进行了调查。本研究的目的是评估PAGE4、SCP-1和SPANXA/D癌睾丸抗原（CTA）基因的表达频率以及一些临床风险标志物，以预测结直肠癌患者的肝转移。使用逆转录聚合酶链反应（RT-PCR）检测法，在90份结直肠肿瘤样本（包括肝转移阴性和阳性肿瘤）中获得了PAGE4、SCP-1和SPANXA/D癌/睾丸抗原（CTA）基因的表达频率。进行统计分析以评估三个研究基因和临床风险因素与结直肠癌肝转移的关联。与无肝转移的原发性肿瘤相比，有肝转移的原发性肿瘤中PAGE4和SCP-1基因的表达频率显著更高（P < 0.05）。在所有研究的临床风险因素中，淋巴结转移和浸润深度与结直肠癌患者的肝转移在统计学上相关。此外，使用多元逻辑回归，我们构建了一个基于PAGE4和淋巴结转移的模型来预测结直肠癌的肝转移。
