Hashim Achu, Mohammed Rafique, Umar Dilshad, Veena Vaswani R, Bahija Basheer, Kusai Baroudi
Department of Forensic Medicine and Toxicology, Yenepoya Medical College, Mangalore, India.
Department of Cleft and Craniofacial Surgery, Yenepoya University, Mangalore, India.
J Adv Pharm Technol Res. 2015 Jul-Sep;6(3):108-13. doi: 10.4103/2231-4040.161506.
Seeds of Strychnos nux-vomica were subjected to preliminary phytochemical tests and its presence was confirmed by thin layer chromatography (TLC) method. The TLC profile of the methanolic extract of seeds of S. nux-vomica was developed using the solvent system toluene:chloroform:methanol in the ratio 8:2:1. The plate was observed in visible light after spraying with Dragendorff's reagent (specific method). High-performance liquid chromatography (HPLC) profile of the methanolic extracts of S. nux-vomica was developed, and the amount of strychnine seems to be 0.36% (w/w) in the seeds. The TLC and HPLC profiles developed are very valuable for the identification of the original drug from their adulterants. The TLC profile identifies the presence of strychnine in the plant material. The quantification method for the strychnine in the seeds can be used for the quality standardization of the raw drug because the strychnine is reported to have some toxicity.
对马钱子种子进行了初步的植物化学测试，并通过薄层色谱（TLC）法确认了其存在。马钱子种子甲醇提取物的TLC图谱是使用甲苯：氯仿：甲醇比例为8:2:1的溶剂系统展开的。喷以碘化铋钾试剂（特定方法）后，在可见光下观察薄板。绘制了马钱子甲醇提取物的高效液相色谱（HPLC）图谱，种子中士的宁含量似乎为0.36%（w/w）。所绘制的TLC和HPLC图谱对于从其掺假物中鉴定原药材非常有价值。TLC图谱可鉴定植物材料中士的宁的存在。种子中士的宁的定量方法可用于生药的质量标准化，因为据报道士的宁具有一定毒性。
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