Gu RuiPing, Zhou Min, Jiang ChunHui, Yu Jian, Xu GeZhi
Department of Ophthalmology and Shanghai Key Laboratory of Visual Impairment and Restoration, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Department of Ophthalmology, No. 5 People's Hospital of Shanghai, Shanghai, China.
Clin Exp Ophthalmol. 2016 Mar;44(2):128-34. doi: 10.1111/ceo.12638. Epub 2015 Dec 8.
We aim to study the level of inflammatory cytokines in aqueous of post-vitrectomy eyes.
Aqueous samples were obtained from post-vitrectomy eyes that underwent cataract surgery. Samples from age-related cataract patients were also collected as controls. The concentrations of cytokines were measured by array-based multiplex sandwich enzyme-linked immunosorbent assay system and microparticle-based multiplex cytokine assay.
Eighteen post-vitrectomy eyes from 18 patients were included; all received one vitrectomy with gas injection for rhegmatogenous retinal detachment. The mean interval between vitrectomy and cataract surgery was 22.33 months (range 6 to 80). Primary results show that in post-vitrectomy eyes the levels of many cytokines, such as B-lymphocyte chemo-attractant, I-309, interleukin (IL)-6, IL-8, interferon γ-induced protein-10 and intercellular adhesion molecule 1, were higher than control. Further analysis demonstrated that the levels of IL-8, interferon γ-induced protein-10 and intercellular adhesion molecule 1 were significantly elevated in post-vitrectomy eyes, even after the subjects were divided by their refractive state.
The concentrations of some cytokines were increased in the anterior chambers of post-vitrectomy eyes even a long time after vitreous surgery. This surge in post-operative cytokines might cause changing in the micro-environment of the anterior, and this might contribute to the development of secondary glaucoma or fasten the development of cataract in these eyes.
我们旨在研究玻璃体切除术后眼房水中炎性细胞因子的水平。
从接受白内障手术的玻璃体切除术后眼中获取房水样本。还收集了年龄相关性白内障患者的样本作为对照。通过基于阵列的多重夹心酶联免疫吸附测定系统和基于微粒的多重细胞因子测定法测量细胞因子的浓度。
纳入了18例患者的18只玻璃体切除术后眼；所有患者均因孔源性视网膜脱离接受了一次玻璃体切除术并注入气体。玻璃体切除术与白内障手术之间的平均间隔时间为22.33个月（范围6至80个月）。初步结果显示，在玻璃体切除术后眼中，许多细胞因子的水平，如B淋巴细胞趋化因子、I-309、白细胞介素（IL）-6、IL-8、干扰素γ诱导蛋白-10和细胞间黏附分子1，均高于对照组。进一步分析表明，即使根据屈光状态对受试者进行分组，玻璃体切除术后眼中IL-8、干扰素γ诱导蛋白-10和细胞间黏附分子1的水平仍显著升高。
即使在玻璃体手术后很长时间，玻璃体切除术后眼的前房中某些细胞因子的浓度仍会升高。术后细胞因子的这种激增可能会导致眼前段微环境的改变，这可能会促使这些眼中继发性青光眼的发生或加速白内障的发展。
