SncRNA715抑制雪旺细胞髓鞘碱性蛋白的合成。

SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.

作者信息

Müller Christina, Hochhaus Nina M, Fontana Xavier, Luhmann Heiko J, White Robin

机构信息

Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.

MRC Laboratory for Molecular Cell Biology and the UCL Cancer Institute, University College London, London, United Kingdom.

出版信息

PLoS One. 2015 Aug 28;10(8):e0136900. doi: 10.1371/journal.pone.0136900. eCollection 2015.

DOI:10.1371/journal.pone.0136900

PMID:26317513

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4552632/

Abstract

Myelin basic proteins (MBP) are major constituents of the myelin sheath in the central nervous system (CNS) and the peripheral nervous system (PNS). In the CNS Mbp translation occurs locally at the axon-glial contact site in a neuronal activity-dependent manner. Recently we identified the small non-coding RNA 715 (sncRNA715) as a key inhibitor of Mbp translation during transport in oligodendrocytes. Mbp mRNA localization in Schwann cells has been observed, but has not been investigated in much detail. Here we could confirm translational repression of Mbp mRNA in Schwann cells. We show that sncRNA715 is expressed and its levels correlate inversely with MBP in cultured Schwann cells and in the sciatic nerve in vivo. Furthermore we could reduce MBP protein levels in cultured Schwann cells by increasing the levels of the inhibitory sncRNA715. Our findings suggest similarities in sncRNA715-mediated translational repression of Mbp mRNA in oligodendrocytes and Schwann cells.

摘要

髓鞘碱性蛋白（MBP）是中枢神经系统（CNS）和周围神经系统（PNS）中髓鞘的主要成分。在中枢神经系统中，Mbp的翻译以神经元活动依赖的方式在轴突-胶质细胞接触部位局部发生。最近，我们鉴定出小非编码RNA 715（sncRNA715）是少突胶质细胞运输过程中Mbp翻译的关键抑制剂。已观察到Mbp mRNA在雪旺细胞中的定位，但尚未进行详细研究。在这里，我们可以证实雪旺细胞中Mbp mRNA的翻译抑制。我们表明，sncRNA715在培养的雪旺细胞和体内坐骨神经中表达，其水平与MBP呈负相关。此外，通过增加抑制性sncRNA715的水平，我们可以降低培养的雪旺细胞中MBP蛋白的水平。我们的研究结果表明，sncRNA715介导的少突胶质细胞和雪旺细胞中Mbp mRNA的翻译抑制具有相似性。