Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; KTH Royal Institute of Technology, School of Biotechnology, Division of Industrial Biotechnology, AlbaNova University Center, 106 91 Stockholm, Sweden.
Curr Opin Biotechnol. 2015 Dec;36:73-84. doi: 10.1016/j.copbio.2015.07.003. Epub 2015 Aug 28.
Recent metabolic engineering strategies for bio-based production of monomers and polymers are reviewed. In the case of monomers, we describe strategies for producing polyamide precursors, namely diamines (putrescine, cadaverine, 1,6-diaminohexane), dicarboxylic acids (succinic, glutaric, adipic, and sebacic acids), and ω-amino acids (γ-aminobutyric, 5-aminovaleric, and 6-aminocaproic acids). Also, strategies for producing diols (monoethylene glycol, 1,3-propanediol, and 1,4-butanediol) and hydroxy acids (3-hydroxypropionic and 4-hydroxybutyric acids) used for polyesters are reviewed. Furthermore, we review strategies for producing aromatic monomers, including styrene, p-hydroxystyrene, p-hydroxybenzoic acid, and phenol, and propose pathways to aromatic polyurethane precursors. Finally, in vivo production of polyhydroxyalkanoates and recombinant structural proteins having interesting applications are showcased.
本文综述了生物基单体和聚合物生产的最新代谢工程策略。在单体方面，我们描述了用于生产聚酰胺前体的策略，即二胺（腐胺、尸胺、1,6-二氨基己烷）、二酸（琥珀酸、戊二酸、己二酸和癸二酸）和ω-氨基酸（γ-氨基丁酸、5-氨基戊酸和 6-氨基己酸）。此外，还综述了用于聚酯的二醇（单乙二醇、1,3-丙二醇和 1,4-丁二醇）和羟基酸（3-羟基丙酸和 4-羟基丁酸）的生产策略。此外，我们还综述了芳香族单体的生产策略，包括苯乙烯、对羟基苯乙烯、对羟基苯甲酸和苯酚，并提出了芳香族聚氨酯前体的途径。最后，展示了具有有趣应用的聚羟基烷酸酯和重组结构蛋白的体内生产。
