利用磁镊在单蛋白水平揭示机械传感机制。

Uncovering mechanosensing mechanisms at the single protein level using magnetic tweezers.

作者信息

Le Shimin, Liu Ruchuan, Lim Chwee Teck, Yan Jie

机构信息

Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Department of Physics, National University of Singapore, Singapore 117542, Singapore; Centre for Bioimaging Sciences, National University of Singapore, Singapore 117546, Singapore.

Department of Physics, National University of Singapore, Singapore 117542, Singapore; College of Physics, Chongqing University, Chongqing 401331, China.

出版信息

Methods. 2016 Feb 1;94:13-8. doi: 10.1016/j.ymeth.2015.08.020. Epub 2015 Aug 28.

DOI:10.1016/j.ymeth.2015.08.020

PMID:26318089

Abstract

Mechanosensing of the micro-environments has been shown to be essential for cell survival, growth, differentiation and migration. The mechanosensing pathways are mediated by a set of mechanosensitive proteins located at focal adhesion and cell-cell adherens junctions as well as in the cytoskeleton network. Here we review the applications of magnetic tweezers on elucidating the molecular mechanisms of the mechanosensing proteins. The scope of this review includes the principles of the magnetic tweezers technology, theoretical analysis of force-dependent stability and interaction of mechanosensing proteins, and recent findings obtained using magnetic tweezers.

摘要

已证明对微环境的机械传感对于细胞存活、生长、分化和迁移至关重要。机械传感途径由位于粘着斑和细胞间粘附连接以及细胞骨架网络中的一组机械敏感蛋白介导。在此，我们综述了磁性镊子在阐明机械传感蛋白分子机制方面的应用。本综述的范围包括磁性镊子技术的原理、机械传感蛋白的力依赖性稳定性和相互作用的理论分析，以及使用磁性镊子获得的最新发现。