Arroyo Mühr Laila Sara, Bzhalava Davit, Lagheden Camilla, Eklund Carina, Johansson Hanna, Forslund Ola, Dillner Joakim, Hultin Emilie
Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
Department of Laboratory Medicine, Lund University, Malmö, Sweden.
Virology. 2015 Nov;485:283-8. doi: 10.1016/j.virol.2015.07.023. Epub 2015 Aug 28.
Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deeper metagenomics sequencing. Sequencing of whole genome amplified DNA from 40 apparently "HPV-negative" condylomata detected HPV in 37/40 specimens. We found 75 different HPV types, out of which 43 represented novel putative HPV types. Three types were cloned and established as HPV types 200, 201 and 202. Molluscum contagiosum virus was detected in 24 of the 40 samples. In summary, deep sequencing enables detection of HPV in almost all condylomata. "HPV-negative" condylomata might largely be explained by clinical misdiagnosis or the presence of viral variants, distantly related HPV types and/or low viral loads.
尖锐湿疣由人乳头瘤病毒（HPV）引起。使用通用引物进行聚合酶链反应（PCR）通常能在超过96%的尖锐湿疣中检测到HPV。宏基因组测序发现，一些“HPV阴性”的尖锐湿疣确实含有HPV。我们希望使用更深入的宏基因组测序对“HPV阴性”的尖锐湿疣进行重新评估。对40例明显“HPV阴性”的尖锐湿疣的全基因组扩增DNA进行测序，在37/40个样本中检测到了HPV。我们发现了75种不同的HPV类型，其中43种代表新的假定HPV类型。三种类型被克隆并确定为HPV 200型、201型和202型。在40个样本中的24个中检测到了传染性软疣病毒。总之，深度测序能够在几乎所有尖锐湿疣中检测到HPV。“HPV阴性”的尖锐湿疣很大程度上可能是由于临床误诊或存在病毒变异体、远缘相关的HPV类型和/或低病毒载量所致。
