Matsubayashi Makoto, Murakoshi Naoko, Komatsu Tetsuya, Tokoro Masaharu, Haritani Makoto, Shibahara Tomoyuki
National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan; Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
Chuou Animal Health and Hygiene Service Centre of Aichi Prefecture, Okazaki, Aichi 444-0805, Japan.
Infect Genet Evol. 2015 Dec;36:8-14. doi: 10.1016/j.meegid.2015.08.030. Epub 2015 Aug 28.
To date, three Entamoeba spp. (E. suis, zoonotic E. polecki and E. histolytica) have been identified in pigs, but their pathogenicity and molecular classification have not been fully determined. Examination and pathological analysis of pigs (n=3) with diarrhoea was conducted and revealed the presence of Entamoeba organisms. We performed a genetic analysis of the isolate using the small-subunit ribosomal RNA (SSU rRNA) gene region to identify the species. A severe ulcerative colitis was observed histopathologically with inflammatory cells, including macrophages and neutrophils, infiltrating the mucous membranes of the cecum and colon. Many Entamoeba trophozoites were found at the erosion site or at ulcerative lesions. Pathogenic viruses or bacteria were not detected. The SSU rRNA sequence of the Entamoeba isolate was found to be completely homologous to that of E. polecki subtype 3.
迄今为止，在猪体内已鉴定出三种内阿米巴属物种（猪内阿米巴、人畜共患的泡状内阿米巴和溶组织内阿米巴），但其致病性和分子分类尚未完全确定。对三头腹泻猪进行了检查和病理分析，发现存在内阿米巴生物体。我们使用小亚基核糖体RNA（SSU rRNA）基因区域对分离株进行了基因分析，以鉴定该物种。组织病理学观察发现严重的溃疡性结肠炎，包括巨噬细胞和中性粒细胞在内的炎症细胞浸润盲肠和结肠黏膜。在糜烂部位或溃疡性病变处发现许多内阿米巴滋养体。未检测到致病性病毒或细菌。发现内阿米巴分离株的SSU rRNA序列与泡状内阿米巴3型亚型的序列完全同源。
