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采用臭氧氧化法与一体化固定膜活性污泥法(IFAS)联合处理油砂加工废水(OSPW)。

Treatment of oil sands process-affected water (OSPW) using ozonation combined with integrated fixed-film activated sludge (IFAS).

机构信息

Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 1H9.

Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 1H9.

出版信息

Water Res. 2015 Nov 15;85:167-76. doi: 10.1016/j.watres.2015.08.019. Epub 2015 Aug 12.

Abstract

Two integrated fixed-film activated sludge (IFAS) reactors were operated continuously to treat raw (untreated) and ozonated (30 mg/L) oil sands process-affected water (OSPW). After 11 months, 12.1% of the acid extractable fraction (AEF) and 43.1% of the parent naphthenic acids (NAs) were removed in the raw OSPW IFAS, while 42.0% AEF and 80.2% of parent NAs were removed in the ozonated OSPW IFAS. UPLC/HRMS analysis showed that NA biodegradation significantly decreased as the NA cyclization number increased. Confocal laser scanning microscopy (CLSM) results showed that the biofilm in the ozonated OSPW IFAS was significantly thicker (94 ± 1.6 μm) than the biofilm in the raw OSPW IFAS (72 ± 2.8 μm) after 283 days of cultivation. The quantitative polymerase chain reaction (q-PCR) revealed that the abundance proportions of both nitrifier genes (AomA, NSR and Nitro) and denitrifier genes (narG, nirS, nirK and nosZ) within total bacteria were significantly higher in biofilms than in flocs in the raw OSPW IFAS system, but a different trend was observed in the ozonated OSPW IFAS system.

摘要

连续运行了两个一体化固定膜生物活性污泥(IFAS)反应器来处理原水(未经处理)和臭氧化(30mg/L)油砂加工废水(OSPW)。经过 11 个月,在原水 OSPW-IFAS 中,酸可萃取部分(AEF)的 12.1%和母体环烷酸(NA)的 43.1%被去除,而在臭氧化 OSPW-IFAS 中,AEF 的 42.0%和母体 NA 的 80.2%被去除。超高效液相色谱/高分辨质谱(UPLC/HRMS)分析表明,随着 NA 环化数的增加,NA 的生物降解显著降低。共聚焦激光扫描显微镜(CLSM)结果表明,经过 283 天的培养,臭氧化 OSPW-IFAS 中的生物膜比原水 OSPW-IFAS 中的生物膜(72±2.8μm)厚得多(94±1.6μm)。定量聚合酶链反应(q-PCR)显示,在原水 OSPW-IFAS 系统中,生物膜中氨氧化菌(AomA、NSR 和 Nitro)和反硝化菌(narG、nirS、nirK 和 nosZ)的丰度比例明显高于絮体中的丰度比例,但在臭氧化 OSPW-IFAS 系统中观察到了不同的趋势。

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