Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China; Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT 06269, USA.
Bioresour Technol. 2015 Dec;197:56-63. doi: 10.1016/j.biortech.2015.08.025. Epub 2015 Aug 14.
Long-term effect of pH (4, 10, and uncontrolled) on short-chain fatty acid (SCFA) accumulation, microbial community and sludge reduction were investigated in waste activated sludge (WAS) fermentors for over 90days. The average SCFAs accumulation was 1721.4 (at pH 10), 114.2 (at pH 4), and 58.1 (at uncontrolled pH) mg chemical oxygen demand (COD)/L. About 31.65mgCOD/L was produced at pH 10, accounting for 20% of the influent COD. Illumina MiSeq sequencing revealed that Alcaligenes (hydrolic bacteria) and Erysipelothrix (acidogenic bacteria) were enriched at pH 10, while less acidogenic bacteria existed at pH 4 than pH 10, and no acidogenic bacteria were detected at the uncontrolled pH. The ratios of archaea to bacteria were 1:41, 1:16, and 1:9 at the pH of 10, 4, and uncontrolled. This study elucidated the effects of pH on WAS fermentation, and established the correlation of microbial structure with SCFAs accumulations and sludge reduction.
在废水活性污泥（WAS）发酵器中，研究了 pH 值（4、10 和未控制）对短链脂肪酸（SCFA）积累、微生物群落和污泥减少的长期影响，时间超过 90 天。平均 SCFA 积累量分别为 1721.4（在 pH 值 10 时）、114.2（在 pH 值 4 时）和 58.1（在未控制的 pH 值时）mg 化学需氧量（COD）/L。在 pH 值 10 时产生了约 31.65mgCOD/L，占进水 COD 的 20%。Illumina MiSeq 测序表明，Alcaligenes（水解细菌）和 Erysipelothrix（产酸细菌）在 pH 值 10 时得到了富集，而在 pH 值 4 时的产酸菌比 pH 值 10 时少，在未控制的 pH 值时则没有检测到产酸菌。在 pH 值为 10、4 和未控制时，古菌与细菌的比例分别为 1:41、1:16 和 1:9。本研究阐明了 pH 值对 WAS 发酵的影响，并建立了微生物结构与 SCFA 积累和污泥减少的相关性。
