Holt H M, Danielsen T K, Justesen U S
Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark.
Eur J Clin Microbiol Infect Dis. 2015 Nov;34(11):2243-6. doi: 10.1007/s10096-015-2475-x. Epub 2015 Aug 30.
Reduced susceptibility to metronidazole and vancomycin in Clostridium difficile has been reported, which emphasises the need for simple antimicrobial susceptibility testing methods. The aim of this study was to apply a published disc diffusion method and zone diameter breakpoint correlates to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) epidemiological minimum inhibitory concentration (MIC) cut-off values in a routine setting. Metronidazole and vancomycin zone diameters from 2702 isolates were recorded. Fifteen isolates had a metronidazole zone diameter below the published breakpoint (<23 mm) and five isolates had a vancomycin zone diameter below the published breakpoint (<19 mm), most of which were polymerase chain reaction (PCR) ribotype 027. The total number of PCR ribotype 027 was 29 (1.1 %). Overall, C. difficile PCR ribotype 027 isolates had smaller zone diameters than non-027 isolates. The disc diffusion method is very simple and inexpensive, and the published zone diameter breakpoints will detect C. difficile isolates with reduced susceptibility to metronidazole and vancomycin.
据报道，艰难梭菌对甲硝唑和万古霉素的敏感性降低，这凸显了对简单的抗菌药物敏感性检测方法的需求。本研究的目的是在常规环境中应用已发表的纸片扩散法以及与欧洲抗菌药物敏感性检测委员会（EUCAST）流行病学最低抑菌浓度（MIC）临界值相关的抑菌圈直径断点。记录了2702株分离株的甲硝唑和万古霉素抑菌圈直径。15株分离株的甲硝唑抑菌圈直径低于已发表的断点（<23毫米），5株分离株的万古霉素抑菌圈直径低于已发表的断点（<19毫米），其中大多数是聚合酶链反应（PCR）核糖体分型027。PCR核糖体分型027的总数为29株（1.1%）。总体而言，艰难梭菌PCR核糖体分型027分离株的抑菌圈直径比非027分离株小。纸片扩散法非常简单且成本低廉，已发表的抑菌圈直径断点将检测出对甲硝唑和万古霉素敏感性降低的艰难梭菌分离株。
