Bogema D R, Fell S A, O'Rourke B A, Collins D, Eamens G J, Jenkins C
The ithree institute, University of Technology Sydney, Ultimo, NSW 2007, Australia; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
Vet Parasitol. 2015 Sep 15;212(3-4):379-81. doi: 10.1016/j.vetpar.2015.08.014. Epub 2015 Aug 20.
Theileria orientalis is an emerging bovine pathogen in Australasia. PCR-based detection methods for this parasite are sensitive but relatively expensive, partly due to costs associated with DNA extraction. An inexpensive and efficient technique was developed for the extraction of T. orientalis DNA from blood based on hypotonic erythrocyte lysis and detergent-proteinase K treatment (DPK method). The DPK method compares favourably to a commercial extraction kit when paired with a T. orientalis multiplex qPCR.
东方泰勒虫是一种在澳大拉西亚地区新出现的牛病原体。针对这种寄生虫基于聚合酶链式反应（PCR）的检测方法灵敏但相对昂贵，部分原因在于与DNA提取相关的成本。基于低渗红细胞裂解和去污剂 - 蛋白酶K处理（DPK方法），开发了一种从血液中提取东方泰勒虫DNA的廉价且高效的技术。当与东方泰勒虫多重定量PCR配对使用时，DPK方法与商业提取试剂盒相比具有优势。
