Teramura Hajime, Iwasaki Mihoko, Ogihara Hirokazu
Yokohama Research Center, JNC Corporation, 5-1, Ookawa, Kanazawa-ku, Yokohama, Kanagawa 236-8605, Japan; Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon Universitys 1866, Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Yokohama Research Center, JNC Corporation, 5-1, Ookawa, Kanazawa-ku, Yokohama, Kanagawa 236-8605, Japan.
J Food Prot. 2015 Sep;78(9):1750-5. doi: 10.4315/0362-028X.JFP-15-115.
The presence of expanded-spectrum β-lactamase (ESBL)-producing Escherichia coli is a common problem in the isolation of Campylobacter from poultry samples using conventional cefoperazone-based selective media. A novel chromogenic medium (CM-HT), based on modified charcoal cefoperazone deoxycholate agar (mCCDA), has been developed as a solution for improved Campylobacter detection from poultry samples. Although the basic components of CM-HT are the same as mCCDA, CM-HT uses both granular charcoal and sodium cefoxitin to enhance viewability and inhibit ESBL-producing bacteria. All tested Campylobacter jejuni (n = 31) and Campylobacter coli (n = 6) strains grew and formed purple-colored colonies on CM-HT. In contrast, the growth of all other tested microorganisms, including ESBL-producing E. coli strains, was suppressed by this medium. Additionally, 84 poultry samples were examined for the presence of Campylobacter using the ISO 10272-1 method (enrichment with Bolton broth) and the NIHSJ-02 method (enrichment with Preston broth) with mCCDA and CM-HT media for the isolation. The numbers of samples from which Camplylobacter was detected on CM-HT using Preston and Bolton broth were 22 and 18, whereas the numbers on mCCDA were 22 and 13, respectively. Only Campylobacter was detected on CM-HT using both enrichment broths; however, there were 5 and 19 samples from which ESBL-producing E. coli was detected on mCCDA using Preston and Bolton broth, respectively. Thus, there was a significant difference between CM-HT and mCCDA in selectivity for ESBL-producing E. coli regardless of which enrichment broth was used. The results obtained demonstrated that CM-HT is a possible solution for the improved isolation of Campylobacter from poultry samples.
在使用传统的基于头孢哌酮的选择性培养基从家禽样本中分离弯曲杆菌时，产超广谱β-内酰胺酶（ESBL）的大肠杆菌的存在是一个常见问题。一种基于改良的木炭头孢哌酮脱氧胆酸盐琼脂（mCCDA）的新型显色培养基（CM-HT）已被开发出来，作为从家禽样本中改进弯曲杆菌检测的解决方案。尽管CM-HT的基本成分与mCCDA相同，但CM-HT同时使用颗粒状木炭和头孢西丁钠来提高可视性并抑制产ESBL的细菌。所有测试的空肠弯曲杆菌（n = 31）和结肠弯曲杆菌（n = 6）菌株在CM-HT上生长并形成紫色菌落。相比之下，包括产ESBL的大肠杆菌菌株在内的所有其他测试微生物的生长都被这种培养基抑制。此外，使用ISO 10272-1方法（用博尔顿肉汤富集）和NIHSJ-02方法（用普雷斯顿肉汤富集），并使用mCCDA和CM-HT培养基进行分离，对84份家禽样本进行了弯曲杆菌检测。使用普雷斯顿肉汤和博尔顿肉汤在CM-HT上检测到弯曲杆菌的样本数量分别为22和18，而在mCCDA上的数量分别为22和13。使用两种富集肉汤在CM-HT上仅检测到弯曲杆菌；然而，分别使用普雷斯顿肉汤和博尔顿肉汤在mCCDA上检测到产ESBL的大肠杆菌的样本有5份和19份。因此，无论使用哪种富集肉汤，CM-HT和mCCDA在对产ESBL的大肠杆菌的选择性上都存在显著差异。获得的结果表明，CM-HT是从家禽样本中改进弯曲杆菌分离的一种可能解决方案。
