同位素标记实验和（13）C 通量分析的应用，以实现合理的途径工程。
Application of isotope labeling experiments and (13)C flux analysis to enable rational pathway engineering.
机构信息
Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA.
Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA.
出版信息
Curr Opin Biotechnol. 2015 Dec;36:50-6. doi: 10.1016/j.copbio.2015.08.004. Epub 2015 Aug 28.
Isotope labeling experiments (ILEs) and (13)C flux analysis provide actionable information for metabolic engineers to identify knockout, overexpression, and/or media optimization targets. ILEs have been used in both academic and industrial labs to increase product formation, discover novel metabolic functions in previously uncharacterized organisms, and enhance the metabolic efficiency of host cell factories. This review highlights specific examples of how ILEs have been used in conjunction with enzyme or metabolic engineering to elucidate host cell metabolism and improve product titer, rate, or yield in a directed manner. We discuss recent progress and future opportunities involving the use of ILEs and (13)C flux analysis to characterize non-model host organisms and to identify and subsequently eliminate wasteful byproduct pathways or metabolic bottlenecks.
同位素标记实验（ILEs）和（13）C 通量分析为代谢工程师提供了可行的信息，以确定敲除、过表达和/或培养基优化的目标。ILEs 已在学术和工业实验室中用于增加产物形成、在以前未表征的生物体中发现新的代谢功能以及提高宿主细胞工厂的代谢效率。这篇综述重点介绍了 ILEs 如何与酶或代谢工程结合使用，以阐明宿主细胞代谢并以定向方式提高产物滴度、速率或产量的具体实例。我们讨论了涉及使用 ILEs 和（13）C 通量分析来表征非模型宿主生物以及识别和随后消除浪费的副产物途径或代谢瓶颈的最新进展和未来机会。
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