Lima Prando Thiago Buno, Barboza Lorena Neris, Gasparotto Francielly Mourão, Araújo Valdinei de Oliveira, Slgnor Tirloni Cleide Adriane, de Souza Lauro Mera, Lourenço Emerson Luiz Botelho, Gasparotto Junior Arquimedes
Laboratório de Farmacologia e Toxicologia de Produtos Naturais, Universidade Paranaense, ,Curitiba, PR Brazil.
Laboratório de Farmacologia Cardiovascular, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, MS, Brasil.
J Ethnopharmacol. 2015 Nov 4;174:369-78. doi: 10.1016/j.jep.2015.08.029. Epub 2015 Aug 28.
Although Echinodorus grandiflorus, Cuphea carthagenensis, and Phyllanthus tenellus infusions are used in Brazilian folk medicine due to their possible diuretic effect, none of these species was critically investigated as a diuretic drug. So, the aim of this study was to evaluate the possible acute diuretic activity of ethanol soluble fractions (ES) obtained from these species and assess the relationship between renal cortical blood flow and their antioxidant and hypotensive activity using normotensive Wistar rats.
The preparation obtained from E. grandiflorus (ES-EG), C. carthagenensis (ES-CC), and P. tenellus (ES-PT) infusions was orally administered in a single dose to rats. Urine excretion rate, pH, density, conductivity and Na(+), K(+), Cl(-) and HCO3(-) contents were measured in the urine of saline-loaded animals. Concentration of electrolytes, total protein, urea, creatinine, and angiotensin converting enzyme (ACE) activity were evaluated in collected serum. The involvement of the renal cortical blood flow and antioxidative activity in the hypotensive and diuretic effects was also determined.
Water and Na(+), Cl(-) and Na(+) excretion rates were significantly increased by ES-EG, while urinary bicarbonate excretion was reduced. Moreover, ES obtained from E. grandiflorus was able to significantly increase renal blood flow and reduce mean arterial pressure and oxidative stress in "in vitro" and "in vivo" models. All other parameters evaluated were not affected by any treatment.
The results presented here shown that the ES-EG obtained from E. grandiflorus leaves shown a significant diuretic and hypotensive activity and suggest that these effects could be related with an important renal and systemic vasodilator effect. In addition, it was shown for the first time that the pharmacological effects of ES obtained from P. tenellus and C. carthagenensis do not support its popular use as a diuretic agent.
尽管大花泽泻、卡塔赫纳萼距花和小叶叶下珠的浸剂因其可能的利尿作用而被用于巴西民间医学，但这些物种均未作为利尿药物进行严格研究。因此，本研究的目的是评估从这些物种中获得的乙醇可溶部分（ES）的可能急性利尿活性，并使用正常血压的Wistar大鼠评估肾皮质血流与其抗氧化和降压活性之间的关系。
将从大花泽泻（ES-EG）、卡塔赫纳萼距花（ES-CC）和小叶叶下珠（ES-PT）浸剂中获得的制剂以单剂量口服给予大鼠。测量盐水负荷动物尿液中的尿排泄率、pH值、密度、电导率以及Na(+)、K(+)、Cl(-)和HCO3(-)含量。评估收集的血清中电解质、总蛋白、尿素、肌酐和血管紧张素转换酶（ACE）活性的浓度。还确定了肾皮质血流和抗氧化活性在降压和利尿作用中的参与情况。
ES-EG显著增加了水以及Na(+)、Cl(-)和Na(+)的排泄率，而尿碳酸氢盐排泄减少。此外，从大花泽泻中获得的ES能够在“体外”和“体内”模型中显著增加肾血流量并降低平均动脉压和氧化应激。评估的所有其他参数均未受任何处理影响。
此处呈现的结果表明，从大花泽泻叶中获得的ES-EG具有显著的利尿和降压活性，并表明这些作用可能与重要的肾和全身血管舒张作用有关。此外，首次表明从小叶叶下珠和卡塔赫纳萼距花中获得的ES的药理作用不支持其作为利尿剂的普遍用途。
