Borai Anwar, Livingstone Callum, Farzal Anwar, Baljoon Dalal, Al Sofyani Abeer, Bahijri Suhad, Kadam Ibrahim, Hafiz Khalid, Abdelaal Mohamed, Ferns Gordon
King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, P.O. Box 9515, Jeddah 21423, Saudi Arabia.
Faculty of Health & Medical Sciences, University of Surrey, GU2 7XH Guildford, United Kingdom.
Clin Biochem. 2016 Jan;49(1-2):51-6. doi: 10.1016/j.clinbiochem.2015.08.023. Epub 2015 Aug 28.
Previous studies have investigated the impact of venesection upon different metabolic indices in patients with various conditions (e.g., type 2 diabetes and iron overload). We aimed to investigate the changes on different metabolic indices including glycemic, iron, lipids and inflammatory markers at different time points after blood donation in male subjects with normal glucose tolerance.
42 male subjects were recruited to the study. Glucose tolerance was assessed by oral glucose tolerance test before (visit A) and after the blood donation (1day, visit B; 1week, visit C; 3weeks, visit D; and 3months, visit E). Fasting glucose, HbA1c, insulin, lipids, uric acid, C-reactive protein, iron stores and insulin resistance (HOMA-IR, ISI-gly) indices were measured. A repeated measures ANOVA was used for comparisons of quantitative variables between different visits.
All subjects had normal glucose tolerance according to WHO criteria. Fasting glucose, insulin and HOMA-IR were significantly higher (2%, p<0.05; ~21%, p<0.01; and ~11%, p<0.05 respectively) at visit B following donation. At visit D, the mean±SE for HbA1c (5.28±0.06%) was significantly lower with a difference in percentage of ~-3% and p<0.05 compared to visit A (5.44±0.06%). Ferritin decreased significantly at visits B, C, D and E (-8%, p<0.01, ~-24%, p<0.001, ~-39%, p<0.001 and ~-29%, p<0.01 respectively), when compared to visit A.
At different time points after blood donation, glycemic status and iron stores are affected significantly in male blood donors with normal glucose tolerance. The changes were particularly evident three weeks after donation. Hence, the interpretation of these parameters in male blood donors needs to take this into account, and the mechanisms resulting in these effects need to be clarified.
以往研究调查了放血疗法对患有各种疾病（如2型糖尿病和铁过载）患者不同代谢指标的影响。我们旨在研究糖耐量正常男性受试者献血后不同时间点血糖、铁、脂质和炎症标志物等不同代谢指标的变化。
招募42名男性受试者参与本研究。在献血前（访视A）和献血后（1天，访视B；1周，访视C；3周，访视D；3个月，访视E）通过口服葡萄糖耐量试验评估糖耐量。测量空腹血糖、糖化血红蛋白、胰岛素、脂质、尿酸、C反应蛋白、铁储备和胰岛素抵抗（HOMA-IR、ISI- gly）指标。采用重复测量方差分析比较不同访视间的定量变量。
根据世界卫生组织标准，所有受试者糖耐量均正常。献血后访视B时，空腹血糖、胰岛素和HOMA-IR显著升高（分别约为2%，p<0.05；约为21%，p<0.01；约为11%，p<0.05）。与访视A（5.44±0.06%）相比，访视D时糖化血红蛋白的均值±标准误（5.28±0.06%）显著降低，百分比差异约为-3%，p<0.05。与访视A相比，访视B、C、D和E时铁蛋白显著降低（分别约为-8%，p<0.01；约为-24%，p<0.001；约为-39%，p<0.001；约为-29%，p<0.01）。
在献血后的不同时间点，糖耐量正常的男性献血者的血糖状态和铁储备受到显著影响。这些变化在献血后三周尤为明显。因此，在解释男性献血者的这些参数时需要考虑到这一点，并且需要阐明导致这些影响的机制。
