Coelho Raquel G, Calaça Isadora C, Celestrini Deborah M, Correia-Carneiro Ana Helena P, Costa Mauricio M, Zancan Patricia, Sola-Penna Mauro
Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
Instituto de Bioquímica Médica Leopoldo De Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
Oncotarget. 2015 Oct 6;6(30):29375-87. doi: 10.18632/oncotarget.4910.
Glycolytic enzymes, such as hexokinase and phosphofructokinase, have been reported to be upregulated in many cancer types. Here, we evaluated these two enzymes in 54 breast cancer samples collected from volunteers subjected to mastectomy, and the results were correlated with the prognosis markers commonly used. We found that both enzymes positively correlate with the major markers for invasiveness and aggressiveness. For invasiveness, the enzymes activities increase in parallel to the tumor size. Moreover, we found augmented activities for both enzymes when the samples were extirpated from patients presenting lymph node involvement or occurrence of metastasis. For aggressiveness, we stained the samples for the estrogen and progesterone receptors, HER-2, p53 and Ki-67. The enzyme activities positively correlated with all markers but Ki-67. Finally, we conclude that these enzymes are good markers for breast cancer prognosis.
据报道，糖酵解酶，如己糖激酶和磷酸果糖激酶，在许多癌症类型中均有上调。在此，我们对54例接受乳房切除术的志愿者所提供的乳腺癌样本中的这两种酶进行了评估，并将结果与常用的预后标志物进行了关联分析。我们发现这两种酶均与侵袭性和恶性程度的主要标志物呈正相关。对于侵袭性而言，酶活性随肿瘤大小的增加而平行升高。此外，当从出现淋巴结受累或转移的患者身上切除样本时，我们发现这两种酶的活性均有所增强。对于恶性程度，我们对样本进行雌激素和孕激素受体、HER-2、p53和Ki-67染色。酶活性与除Ki-67之外的所有标志物均呈正相关。最后，我们得出结论，这些酶是乳腺癌预后的良好标志物。
